Sequence and transfection of BoLA-DRB3 cDNAs

G C Russell, D C Fraser, S Craigmile, R A Oliver, B M Dutia, E J Glass

Research output: Contribution to journalArticlepeer-review

Abstract

Bovine MHC (BoLA-) DRB3 alleles encoded by the DH8A, DH22A and DH24A class II haplotypes were cloned from cDNA and characterized by sequence analysis. Comparison with other full-length DRB3 sequences suggested that DRB3 alleles may have evolved through multiple lineages. All three BoLA-DRB3 alleles were shown to express on the surface of transfected cells, and the transfectants were used to define or confirm the class II specificity of a panel of monoclonal antibodies.
Original languageEnglish
Pages (from-to)219-22
Number of pages4
JournalAnimal Genetics
Volume31
Issue number3
Publication statusPublished - Jun 2000

Keywords

  • Alleles
  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal
  • Antibody Specificity
  • Cattle
  • DNA, Complementary
  • Evolution, Molecular
  • Genes, MHC Class II
  • HLA-DR Antigens
  • HLA-DRB3 Chains
  • Haplotypes
  • Histocompatibility Antigens Class II
  • Molecular Sequence Data
  • Sequence Analysis, Protein
  • Sequence Homology, Amino Acid
  • Transfection

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