Abstract / Description of output
The pentasaccharide (XXG),
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obtained from Rosa xyloglucan, was converted to two isomeric tetrasaccharides, a and b (Xyl(1) . Glc(3)), by mild acid hydrolysis. During hydrolysis in 2 M trifluoroacetic acid at 90 degrees C, optimal yields of a and b were obtained after 20-40 min. Each tetrasaccharide was purified by preparative paper chromatography and high-pressure liquid chromatography (HPLC). The two isomers were distinguished by the products of their partial digestion with Driselase, which hydrolyses the glucosidic bonds sequentially from the non-reducing terminus: a and b yielded cellobiose and Xyl --> Glc --> Glc, respectively, showing that they were
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respectively. Tetrasaccharide b was chromatographically identical, upon HPLC on Dionex CarboPac PA1, with the tetrasaccharide produced from XXG by the action of Tropaeolum alpha-D-xylosidase, supporting the proposed structure. Xyloglucan oligosaccharides were assayed quantitatively by measurement of the yield of isoprimeverose (Xyl --> Glc) after complete Driselase digestion.
| Original language | English |
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| Pages (from-to) | 285-293 |
| Number of pages | 9 |
| Journal | Carbohydrate Research |
| Volume | 263 |
| Issue number | 2 |
| Publication status | Published - 17 Oct 1994 |