SEQUENCING OF XYLOGLUCAN OLIGOSACCHARIDES BY PARTIAL DRISELASE DIGESTION - THE PREPARATION AND QUANTITATIVE AND QUALITATIVE-ANALYSIS OF 2 NEW TETRASACCHARIDES

E P LORENCES, Stephen Fry

Research output: Contribution to journalArticlepeer-review

Abstract

The pentasaccharide (XXG),

[GRAPHICS]

obtained from Rosa xyloglucan, was converted to two isomeric tetrasaccharides, a and b (Xyl(1) . Glc(3)), by mild acid hydrolysis. During hydrolysis in 2 M trifluoroacetic acid at 90 degrees C, optimal yields of a and b were obtained after 20-40 min. Each tetrasaccharide was purified by preparative paper chromatography and high-pressure liquid chromatography (HPLC). The two isomers were distinguished by the products of their partial digestion with Driselase, which hydrolyses the glucosidic bonds sequentially from the non-reducing terminus: a and b yielded cellobiose and Xyl --> Glc --> Glc, respectively, showing that they were

[GRAPHICS]

respectively. Tetrasaccharide b was chromatographically identical, upon HPLC on Dionex CarboPac PA1, with the tetrasaccharide produced from XXG by the action of Tropaeolum alpha-D-xylosidase, supporting the proposed structure. Xyloglucan oligosaccharides were assayed quantitatively by measurement of the yield of isoprimeverose (Xyl --> Glc) after complete Driselase digestion.

Original languageEnglish
Pages (from-to)285-293
Number of pages9
JournalCarbohydrate Research
Volume263
Issue number2
Publication statusPublished - 17 Oct 1994

Fingerprint

Dive into the research topics of 'SEQUENCING OF XYLOGLUCAN OLIGOSACCHARIDES BY PARTIAL DRISELASE DIGESTION - THE PREPARATION AND QUANTITATIVE AND QUALITATIVE-ANALYSIS OF 2 NEW TETRASACCHARIDES'. Together they form a unique fingerprint.

Cite this