Serially coupling hydrophobic interaction and reversed-phase chromatography with simultaneous gradients provides greater coverage of the metabolome

Jennifer Haggarty, Madalina Oppermann, Matthew J Dalby, Richard J Burchmore, Ken Cook, Stefan Weidt, Karl EV Burgess

Research output: Contribution to journalArticlepeer-review

Abstract

The serial coupling of a reversed-phase liquid chromatography (RPLC) column to a hydrophilic interaction liquid chromatography (HILIC) column has been developed in recent years for the detection of polar and nonpolar metabolites. TCA intermediates, bile acid standards and numerous polar and non-polar metabolites extracted from beer were analysed using a combined RPLC/HILIC method. Non-polar metabolites were retained by the RPLC column. Polar metabolites not retained by the RPLC column were retained and separated by the HILIC column. The results from this study validate this simple yet powerful metabolomics approach.
Original languageEnglish
Pages (from-to)1465-1470
Number of pages6
JournalMetabolomics
Volume11
Issue number5
DOIs
Publication statusPublished - 11 Jan 2015

Keywords

  • HILIC
  • reversed phase
  • LC-MS
  • organic acids
  • bile acids
  • beer

Fingerprint

Dive into the research topics of 'Serially coupling hydrophobic interaction and reversed-phase chromatography with simultaneous gradients provides greater coverage of the metabolome'. Together they form a unique fingerprint.

Cite this