Seroreactivity to Plasmodium falciparum erythrocyte membrane protein 1 intracellular domain in malaria-exposed children and adults

Mark A Travassos; Amadou Niangaly; Jason A Bailey; Amed Ouattara; Drissa Coulibaly; Matthew B Laurens; Jozelyn Pablo; Algis Jasinskas; Rie Nakajima-Sasaki; Andrea A Berry; Shannon Takala-Harrison; Bourema Kouriba; J Alexandra Rowe; Kirsten E Lyke; Ogobara K Doumbo; Mahamadou A Thera; Philip L Felgner; Christopher V Plowe

doi:10.1093/infdis/jit339

Seroreactivity to Plasmodium falciparum erythrocyte membrane protein 1 intracellular domain in malaria-exposed children and adults

Mark A Travassos, Amadou Niangaly, Jason A Bailey, Amed Ouattara, Drissa Coulibaly, Matthew B Laurens, Jozelyn Pablo, Algis Jasinskas, Rie Nakajima-Sasaki, Andrea A Berry, Shannon Takala-Harrison, Bourema Kouriba, J Alexandra Rowe, Kirsten E Lyke, Ogobara K Doumbo, Mahamadou A Thera, Philip L Felgner, Christopher V Plowe

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) antigens mediate parasite sequestration and host immune evasion. Reactivity to 21 PfEMP1 fragments on a protein microarray was measured in serum samples from Malian children aged 1-6 years and adults. Seroreactivity to PfEMP1 fragments was higher in adults than in children; intracellular conserved fragments were more widely recognized than were extracellular hypervariable fragments. Over a malaria season, children maintained this differential seroreactivity and recognized additional intracellular PfEMP1 fragments. This approach has the potential to identify conserved, seroreactive extracellular PfEMP1 domains critical for protective immunity to malaria.

Original language	English
Pages (from-to)	1514-1519
Number of pages	6
Journal	The Journal of Infectious Diseases
Volume	208
Issue number	9
Early online date	30 Jul 2013
DOIs	https://doi.org/10.1093/infdis/jit339
Publication status	Published - 1 Nov 2013

Keywords / Materials (for Non-textual outputs)

Adult
Antibodies, Protozoan
Antigens, Protozoan
Case-Control Studies
Child
Child, Preschool
Humans
Infant
Malaria, Falciparum
Peptide Fragments
Plasmodium falciparum
Protein Array Analysis
Protein Structure, Tertiary
Protozoan Proteins
Journal Article
malaria
var genes
PfEMP1
immunity
seroreactivity
microarray

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10.1093/infdis/jit339Licence: Creative Commons: Attribution (CC-BY)

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Travassos, M. A., Niangaly, A., Bailey, J. A., Ouattara, A., Coulibaly, D., Laurens, M. B., Pablo, J., Jasinskas, A., Nakajima-Sasaki, R., Berry, A. A., Takala-Harrison, S., Kouriba, B., Rowe, J. A., Lyke, K. E., Doumbo, O. K., Thera, M. A., Felgner, P. L., & Plowe, C. V. (2013). Seroreactivity to Plasmodium falciparum erythrocyte membrane protein 1 intracellular domain in malaria-exposed children and adults. The Journal of Infectious Diseases, 208(9), 1514-1519. https://doi.org/10.1093/infdis/jit339

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title = "Seroreactivity to Plasmodium falciparum erythrocyte membrane protein 1 intracellular domain in malaria-exposed children and adults",

abstract = "Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) antigens mediate parasite sequestration and host immune evasion. Reactivity to 21 PfEMP1 fragments on a protein microarray was measured in serum samples from Malian children aged 1-6 years and adults. Seroreactivity to PfEMP1 fragments was higher in adults than in children; intracellular conserved fragments were more widely recognized than were extracellular hypervariable fragments. Over a malaria season, children maintained this differential seroreactivity and recognized additional intracellular PfEMP1 fragments. This approach has the potential to identify conserved, seroreactive extracellular PfEMP1 domains critical for protective immunity to malaria.",

keywords = "Adult, Antibodies, Protozoan, Antigens, Protozoan, Case-Control Studies, Child, Child, Preschool, Humans, Infant, Malaria, Falciparum, Peptide Fragments, Plasmodium falciparum, Protein Array Analysis, Protein Structure, Tertiary, Protozoan Proteins, Journal Article, malaria, var genes, PfEMP1, immunity, seroreactivity, microarray",

author = "Travassos, {Mark A} and Amadou Niangaly and Bailey, {Jason A} and Amed Ouattara and Drissa Coulibaly and Laurens, {Matthew B} and Jozelyn Pablo and Algis Jasinskas and Rie Nakajima-Sasaki and Berry, {Andrea A} and Shannon Takala-Harrison and Bourema Kouriba and Rowe, {J Alexandra} and Lyke, {Kirsten E} and Doumbo, {Ogobara K} and Thera, {Mahamadou A} and Felgner, {Philip L} and Plowe, {Christopher V}",

year = "2013",

month = nov,

day = "1",

doi = "10.1093/infdis/jit339",

language = "English",

volume = "208",

pages = "1514--1519",

journal = "The Journal of Infectious Diseases",

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Travassos, MA, Niangaly, A, Bailey, JA, Ouattara, A, Coulibaly, D, Laurens, MB, Pablo, J, Jasinskas, A, Nakajima-Sasaki, R, Berry, AA, Takala-Harrison, S, Kouriba, B, Rowe, JA, Lyke, KE, Doumbo, OK, Thera, MA, Felgner, PL & Plowe, CV 2013, 'Seroreactivity to Plasmodium falciparum erythrocyte membrane protein 1 intracellular domain in malaria-exposed children and adults', The Journal of Infectious Diseases, vol. 208, no. 9, pp. 1514-1519. https://doi.org/10.1093/infdis/jit339

TY - JOUR

T1 - Seroreactivity to Plasmodium falciparum erythrocyte membrane protein 1 intracellular domain in malaria-exposed children and adults

AU - Travassos, Mark A

AU - Niangaly, Amadou

AU - Bailey, Jason A

AU - Ouattara, Amed

AU - Coulibaly, Drissa

AU - Laurens, Matthew B

AU - Pablo, Jozelyn

AU - Jasinskas, Algis

AU - Nakajima-Sasaki, Rie

AU - Berry, Andrea A

AU - Takala-Harrison, Shannon

AU - Kouriba, Bourema

AU - Rowe, J Alexandra

AU - Lyke, Kirsten E

AU - Doumbo, Ogobara K

AU - Thera, Mahamadou A

AU - Felgner, Philip L

AU - Plowe, Christopher V

PY - 2013/11/1

Y1 - 2013/11/1

N2 - Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) antigens mediate parasite sequestration and host immune evasion. Reactivity to 21 PfEMP1 fragments on a protein microarray was measured in serum samples from Malian children aged 1-6 years and adults. Seroreactivity to PfEMP1 fragments was higher in adults than in children; intracellular conserved fragments were more widely recognized than were extracellular hypervariable fragments. Over a malaria season, children maintained this differential seroreactivity and recognized additional intracellular PfEMP1 fragments. This approach has the potential to identify conserved, seroreactive extracellular PfEMP1 domains critical for protective immunity to malaria.

AB - Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) antigens mediate parasite sequestration and host immune evasion. Reactivity to 21 PfEMP1 fragments on a protein microarray was measured in serum samples from Malian children aged 1-6 years and adults. Seroreactivity to PfEMP1 fragments was higher in adults than in children; intracellular conserved fragments were more widely recognized than were extracellular hypervariable fragments. Over a malaria season, children maintained this differential seroreactivity and recognized additional intracellular PfEMP1 fragments. This approach has the potential to identify conserved, seroreactive extracellular PfEMP1 domains critical for protective immunity to malaria.

KW - Adult

KW - Antibodies, Protozoan

KW - Antigens, Protozoan

KW - Case-Control Studies

KW - Child

KW - Child, Preschool

KW - Humans

KW - Infant

KW - Malaria, Falciparum

KW - Peptide Fragments

KW - Plasmodium falciparum

KW - Protein Array Analysis

KW - Protein Structure, Tertiary

KW - Protozoan Proteins

KW - Journal Article

KW - malaria

KW - var genes

KW - PfEMP1

KW - immunity

KW - seroreactivity

KW - microarray

U2 - 10.1093/infdis/jit339

DO - 10.1093/infdis/jit339

M3 - Article

C2 - 23901079

SN - 0022-1899

VL - 208

SP - 1514

EP - 1519

JO - The Journal of Infectious Diseases

JF - The Journal of Infectious Diseases

IS - 9

ER -

Seroreactivity to Plasmodium falciparum erythrocyte membrane protein 1 intracellular domain in malaria-exposed children and adults

Abstract

Keywords / Materials (for Non-textual outputs)

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