SERS microsensors for pH measurements in the lumen and ECM of stem cell derived human airway organoids

William H. Skinner, Nicola Robinson, Gareth R. Hardisty, Holly Fleming, Ailsa Geddis, Mark Bradley, Robert D. Gray, Colin J. Campbell

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Patient derived organoids have the potential to improve the physiological relevance of in vitro disease models. However, the 3D architecture of these self-assembled cellular structures makes probing their biochemistry more complex than in traditional 2D culture. We explore the application of surface enhanced Raman scattering microsensors (SERS-MS) to probe local pH gradients within patient derived airway organoid cultures. SERS-MS consist of solid polymer cores decorated with surface immobilised gold nanoparticles which are functionalised with pH sensitive reporter molecule 4-mercaptobenzoic acid (MBA). We demonstrate that by mixing SERS-MS into the extracellular matrix (ECM) of airway organoid cultures the probes can be engulfed by expanding organoids and report on local pH in the organoid lumen and ECM.
Original languageEnglish
JournalChemical Communications
Early online date14 Feb 2023
DOIs
Publication statusE-pub ahead of print - 14 Feb 2023

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