Projects per year
Abstract / Description of output
Patient derived organoids have the potential to improve the physiological relevance of in vitro disease models. However, the 3D architecture of these self-assembled cellular structures makes probing their biochemistry more complex than in traditional 2D culture. We explore the application of surface enhanced Raman scattering microsensors (SERS-MS) to probe local pH gradients within patient derived airway organoid cultures. SERS-MS consist of solid polymer cores decorated with surface immobilised gold nanoparticles which are functionalised with pH sensitive reporter molecule 4-mercaptobenzoic acid (MBA). We demonstrate that by mixing SERS-MS into the extracellular matrix (ECM) of airway organoid cultures the probes can be engulfed by expanding organoids and report on local pH in the organoid lumen and ECM.
Original language | English |
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Journal | Chemical Communications |
Early online date | 14 Feb 2023 |
DOIs | |
Publication status | E-pub ahead of print - 14 Feb 2023 |
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Dive into the research topics of 'SERS microsensors for pH measurements in the lumen and ECM of stem cell derived human airway organoids'. Together they form a unique fingerprint.Projects
- 1 Finished
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CDT-ISM: EPSRC Centre for Doctoral Training in Intelligent Sensing and Mesurementy
Harvey, A., Underwood, I., Walton, A. & Mount, A.
1/04/14 → 15/09/23
Project: Research