Sex-specific marker and trans-zeatin ribosidase in female annual Mercury

Z Yang, J El Aidi, T Ait-Ali, C Augur, G Teller, F Schoentgen, R Durand, B Durand*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Mercurialis annua is dioecious with unisexual flowers lacking vestiges of opposite sex organs. Previously, we showed that the cytokinin trans-zeatin (t-Z) was correlated with female sex genes whereas t-Z production stops at its precursor, the trans-zeatin riboside (t-ZR) in males. Hoping to further relate sex genes and t-Z production, we underwent a search for eventual female-specific DNA and for a specific enzyme producing the feminizing signal. We report the cloning of a female-specific fragment and the identification of a t-ZR beta-ribosidase. The specific-DNA was isolated by AP-PCR (arbitrary primed-polymerase chain reaction) from a female line. Its sequence shows significant identity with the precursor of the beta-subunit of polygalacturonase (PG) isoenzymel cDNA expressed during tomato fruit development. A stereospecific t-ZR beta-ribosidase activity was purified 490-fold from female apices. It belongs to a female non-covalent enzymatic complex which contains a fructose 1-6 biphosphate aldolase and two 14-3-3 proteins (N-terminal sequences). Furthermore, a cytokinin-oxidase(s) and a PG activity were associated to the purified complex. Together, these results suggest that one PG subunit could be the product of the female-specific DNA which, consequently, cannot be a feminizing allele. Complete purification of the t-ZR ribosidase will enable us to further investigate female sex genes and female-marker relationships. (C) 1998 Published by Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)93-103
Number of pages11
JournalPlant science
Volume139
Issue number1
Publication statusPublished - 11 Dec 1998

Keywords

  • sex-specific DNA
  • female-enzymatic complex
  • cytokinin metabolism
  • sex determination
  • Annual Mercury
  • GENE-EXPRESSION
  • IDENTIFICATION
  • FEATURES
  • BINDING

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