Sexing whole human pre-embryos by in-situ hybridization with a Y-chromosome specific DNA probe

J D West, J R Gosden, R R Angell, K M West, A F Glasier, S S Thatcher, D T Baird

Research output: Contribution to journalArticlepeer-review

Abstract

We have used DNA-DNA in-situ hybridization with a DNA probe for the human Y-chromosome to distinguish between male and female human pre-embryos. Both biotinylated and tritiated Y-probes worked well on control cell cultures where 100 interphase nuclei were scored. Morphologically normal pre-embryos could be sexed with confidence with the tritiated Y-probe but the biotin results were less reliable (although only a few pre-embryos were analysed in this way). Early cleavage stage pre-embryos had large nuclei with relatively diffuse Y-bodies and were more difficult to score with the biotinylated Y-probe. Morphologically abnormal pre-embryos often had large nuclei with multiple Y-bodies (presumably polyploid nuclei) or small nuclei with no Y-bodies (possibly fragmenting nuclei). In all, 38 cleaving and two non-cleaving pre-embryos were analysed. The incidence of false positive and false negative cells seen after hybridization of tritiated Y-probes to control lymphocyte cultures suggests that it should normally be possible to distinguish morphologically normal male and female pre-embryos with samples of three to six interphase nuclei.

Original languageEnglish
Pages (from-to)1010-9
Number of pages10
JournalHuman Reproduction
Volume3
Issue number8
Publication statusPublished - Nov 1988

Keywords

  • Blastocyst
  • DNA Probes
  • Female
  • Humans
  • Male
  • Nucleic Acid Hybridization
  • Sex Determination Analysis
  • Y Chromosome

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