Sexually dimorphic expression of secreted frizzled-related (SFRP) genes in the developing mouse Mullerian duct

S Cox, L Smith, D Bogani, M Cheeseman, P Siggers, A Greenfield

Research output: Contribution to journalArticlepeer-review

Abstract

In developing male embryos, the female reproductive tract primordia (Mullerian ducts) regress due to the production of testicular anti-Mullerian hormone (AMH). Because of the association between secreted frizzled-related proteins (SFRPs) and apoptosis, their reported developmental expression patterns and the role of WNT signaling in female reproductive tract development, we examined expression of Sfrp2 and Sfrp5 during development of the Mullerian duct in male (XY) and female (XX) mouse embryos. We show that expression of both Sfrp2 and Sfrp5 is dynamic and sexually dimorphic. In addition, the male-specific expression observed for both genes prior to the onset of regression is absent in mutant male embryos that fail to undergo Mullerian duct regression. We identified ENU-induced point mutations in Sfrp5 and Sfrp2 that are predicted to severely disrupt the function of these genes. Male embryos and adults homozygous for these mutations, both individually and in combination, are viable and apparently fertile with no overt abnormalities of reproductive tract development.

Original languageEnglish
Pages (from-to)1008-1016
Number of pages9
JournalMolecular Reproduction and Development
Volume73
Issue number8
DOIs
Publication statusPublished - Aug 2006

Keywords

  • Mullerian duct regression
  • FEMALE REPRODUCTIVE-TRACT
  • BETA-CATENIN
  • CELL-DEATH
  • mouse sexual development
  • ENU mutagenesis
  • WNT LIGANDS
  • mesonephros
  • SIGNALING PATHWAY
  • DIFFERENTIATION
  • TRANSDUCTION PATHWAY
  • APOPTOSIS
  • SFRP
  • PROTEINS
  • SPEMANN ORGANIZER

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