Projects per year
Abstract
Since its discovery in mid-20th century, the sensitivity of Nuclear Magnetic Resonance (NMR) has increased steadily, in part due to the design of new, sophisticated NMR experiments. Here we report on a liquid-state NMR methodology that significantly increases the sensitivity of diffusion coefficient measurements of pure compounds, allowing to estimate their sizes using a much reduced amount of material. In this method, the diffusion coefficients are being measured by analysing narrow and intense singlets, which are invariant to magnetic field inhomogeneities. The singlets are obtained through signal acquisition embedded in short (<0.5 ms) spin-echo intervals separated by non-selective 180° or 90° pulses, suppressing the chemical shift evolution of resonances and their splitting due to J couplings. The achieved 10−100 sensitivity enhancement results in a 100−10000-fold time saving. Using high field cryoprobe NMR spectrometers, this makes it possible to measure a diffusion coefficient of a medium-size organic molecule in a matter of minutes with as little as a few hundred nanograms of material.
Original language | English |
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Article number | 4410 |
Journal | Nature Communications |
Volume | 14 |
Issue number | 1 |
Early online date | 21 Jul 2023 |
DOIs | |
Publication status | E-pub ahead of print - 21 Jul 2023 |
Fingerprint
Dive into the research topics of 'SHARPER-DOSY: Sensitivity enhanced diffusion-ordered NMR spectroscopy'. Together they form a unique fingerprint.Projects
- 2 Finished
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SHARPER NMR: fast and accurate analysis of molecules, reactions and processes
1/03/19 → 28/11/22
Project: Research
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A National Network for Applications of High-Field NMR in the Life and Physical Sciences
Uhrin, D., Barlow, P., Bell, N. & Bramham, J.
1/05/18 → 30/04/21
Project: Research
Datasets
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SHARPER-DOSY: Sensitivity Enhanced Diffusion-Ordered NMR Spectroscopy
Boaler, P. (Creator), Uhrin, D. (Creator) & Peat, G. (Creator), Edinburgh DataShare, 31 Aug 2023
DOI: 10.7488/ds/7472
Dataset