Sheep (Ovis aries) Macrophage Migration Inhibitory Factor: Molecular cloning, characterization, tissue distribution and expression in the ewe reproductive tract and in the placenta

Federica Lopes, Alessandro Vannoni, Silvia Sestini, Alessandra Casciaro, Antonietta Carducci, Sabrina Bartolommei, Paola Toschi, Grazyna Ptak, Marcella Cintorino, Felice Arcuri*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Macrophage Migration Inhibitory Factor (MIF) is a pivotal regulator of innate and acquired immunity affecting the response and behavior of macrophages and lymphocytes. However, a number of studies indicated wider physiological functions for this cytokine to include key-roles in reproductive biology. The present study was designed to clone the coding sequence of sheep MIF, to examine the characteristics of the protein in vitro, and to evaluate its expression in sheep tissues and in the ewe reproductive tract in vivo. Ovine MIF cDNA consisted of 348 nucleotides encoding a 115 amino acids protein with an estimated molecular mass of 12,343 Da and an isoelectric point of 7.68. Sheep MIF shared high amino acid identity with the other mammalian MIF family members and showed parallel functions to human MIF, displaying enzymatic oxoreductase activity and inducing monocyte transmigration. Expression studies detected a MIF transcript in all the sheep tissues examined. Among reproductive tissues, MIF mRNA and protein were detected in the ovary, oviduct, uterus and placenta. These results indicate that sheep MIF shares crucial features with other MIF family members and delineate its potential involvement in several aspects of ovine physiology. (C) 2011 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)315-323
Number of pages9
JournalCytokine
Volume54
Issue number3
DOIs
Publication statusPublished - Jun 2011

Keywords / Materials (for Non-textual outputs)

  • Molecular cloning
  • Macrophage Migration Inhibitory Factor
  • Cytokine
  • Sheep
  • Reproduction
  • NATURAL-KILLER-CELLS
  • ENDOMETRIAL STROMAL CELLS
  • FETAL-MATERNAL INTERFACE
  • NF-KAPPA-B
  • CHEMOKINE RECEPTORS
  • IMMUNE PRIVILEGE
  • EARLY-PREGNANCY
  • FACTOR MIF
  • CYTOKINES
  • PROGESTERONE

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