Short communication: Mouse mammary tumor virus driven α-lactalbumin expression effects on lactation and fertility of transgenic mice

S Le Guillou, G Tilly, B Passet, L Lefèvre, M Vilotte, J Costa, F Le Provost, J-L Vilotte

Research output: Contribution to journalArticlepeer-review

Abstract

α-Lactalbumin (Alac) is one of the major milk proteins. Its gene expression is restricted to epithelial cells of the lactating mammary gland. The Alac interaction with a uridine 5'-diphosphate-galactosyltransferase induces lactose synthesis, a major osmotic regulator of milk secretion. Other functions attributed to this protein include induction of apoptosis and anti-inflammatory activities. To assess if forced expression of this gene during early gestation or involution could affect mammary physiology, an Alac-encoding minigene was expressed in transgenic mice under the transcriptional regulation of the mouse mammary tumor virus promoter. The mammary expression did not interfere with gestation, resulted in a slight increase in milk yield as indirectly assessed by the 11% increased growth rate of the pups reared by transgenic females compared with that of those reared by control mice, and induced a slight delay in the early involution process, as demonstrated by histological analyses. The use of the mouse mammary tumor virus promoter resulted in Alac expression in several nonmammary tissues, such as the brain, the testis, the ovary, and the uterus. Although it did not affect male reproductive performances, it induced a female subfertile phenotype, characterized by embryonic implantation failure in the transgenic female reproductive tract.

Original languageEnglish
Pages (from-to)2418-24
Number of pages7
JournalJournal of Dairy Science
Volume94
Issue number5
DOIs
Publication statusPublished - May 2011
Externally publishedYes

Keywords

  • Animals
  • Female
  • Fertility
  • Gene Expression
  • Lactalbumin
  • Lactation
  • Male
  • Mammary Glands, Animal
  • Mammary Tumor Virus, Mouse
  • Mice
  • Mice, Transgenic
  • Promoter Regions, Genetic

Fingerprint

Dive into the research topics of 'Short communication: Mouse mammary tumor virus driven α-lactalbumin expression effects on lactation and fertility of transgenic mice'. Together they form a unique fingerprint.

Cite this