TY - JOUR
T1 - Shotgun metagenomic sequencing of the first case of monkeypox virus in Brazil, 2022
AU - Claro, Ingra Morales
AU - Romano, Camila Malta
AU - Candido, Darlan da Silva
AU - Lima, Evelyn Lepka de
AU - Lindoso, José Angelo Lauletta
AU - Ramundo, Mariana Severo
AU - Moreira, Filipe Romero Rebello
AU - Barra, Luiz Alberto Costa
AU - Borges, Luciana Marques Sansão
AU - Medeiros, Lucas Alberto
AU - Tomishige, Marcia Y S
AU - Moutinho, Tomas
AU - Silva, Anderson José Dias da
AU - Rodrigues, Camila Cristina Martini
AU - Azevedo, Luiz Cesar Fernandes de
AU - Villas-Boas, Lucy Santos
AU - Silva, Camila Alves Maia da
AU - Coletti, Thaís Moura
AU - Manuli, Erika R
AU - O'Toole, Aine
AU - Quick, Joshua
AU - Loman, Nicholas
AU - Rambaut, Andrew
AU - Faria, Nuno R
AU - Figueiredo-Mello, Claudia
AU - Sabino, Ester Cerdeira
N1 - Funding Information:
This study was supported by the Medical Research Council-São Paulo Research Foundation (FAPESP) CADDE partnership award (MR/S0195/1 and FAPESP18/143890). We acknowledge support from the Wellcome Trust and Royal Society (Sir Henry Dale Fellowship 204311/Z/16/Z), Bill & Melinda Gates Foundation (INV-034540 and INV-034652), the Wellcome ARTIC network (206298), the Rede Corona-ômica BR MCTI/FINEP affiliated to RedeVírus/MCTI (FINEP 01.20.0029.000462/20, CNPq 404096/2020-4) and CNPq/MCTI funding 402794/2020-6.
Publisher Copyright:
© 2022, Instituto de Medicina Tropical de Sao Paulo. All rights reserved.
PY - 2022/6/24
Y1 - 2022/6/24
N2 - Monkeypox virus (MPXV), a zoonotic virus endemic to the African continent, has been reported in 33 non-endemic countries since May 2022. We report an almost complete genome of the first confirmed case of MPXV in Brazil. Shotgun metagenomic sequencing was completed in 18 hours, from DNA extraction to consensus sequence generation.
AB - Monkeypox virus (MPXV), a zoonotic virus endemic to the African continent, has been reported in 33 non-endemic countries since May 2022. We report an almost complete genome of the first confirmed case of MPXV in Brazil. Shotgun metagenomic sequencing was completed in 18 hours, from DNA extraction to consensus sequence generation.
KW - viral metagenomics
KW - monkeypox virus
KW - West African clade
KW - genomic surveillance
KW - Brazil
UR - https://doi.org/10.48331/scielodata.KOUBE9
U2 - 10.1590/S1678-9946202264048
DO - 10.1590/S1678-9946202264048
M3 - Article
C2 - 35749419
SN - 0036-4665
VL - 64
JO - Revista do Instituto de Medicina Tropical de Sao Paulo
JF - Revista do Instituto de Medicina Tropical de Sao Paulo
ER -