Shulin packages axonemal outer dynein arms for ciliary targeting

Girish R. Mali, Ferdos Abid Ali, Clinton K. Lau, Farida Begum, Jérôme Boulanger, Jonathan D. Howe, Zhuo A. Chen, Juri Rappsilber, Mark Skehel, Andrew P. Carter*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The main force generators in eukaryotic cilia and flagella are axonemal outer dynein arms (ODAs). During ciliogenesis, these ~1.8-megadalton complexes are assembled in the cytoplasm and targeted to cilia by an unknown mechanism. Here, we used the ciliate Tetrahymena to identify two factors (Q22YU3 and Q22MS1) that bind ODAs in the cytoplasm and are required for ODA delivery to cilia. Q22YU3, which we named Shulin, locked the ODA motor domains into a closed conformation and inhibited motor activity. Cryo-electron microscopy revealed how Shulin stabilized this compact form of ODAs by binding to the dynein tails. Our findings provide a molecular explanation for how newly assembled dyneins are packaged for delivery to the cilia.

Original languageEnglish
Article numberabe0526
Pages (from-to)910-916
Number of pages7
JournalScience
Volume371
Issue number6532
DOIs
Publication statusPublished - 26 Feb 2021

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