Signatures of selection for bonamiosis resistance in European flat oyster (Ostrea edulis): new genomic tools for breeding programs and management of natural resources

Manuel Vera, B.G. Pardo , Asuncion Cao, Román Vilas, Carlos Fernandez , Andres Blanco, Alejandro Gutierrez, Timothy Bean, Ross Houston, Antonio Villalba, Paulino Martinez

Research output: Contribution to journalArticlepeer-review

Abstract

The European flat oyster (Ostrea edulis) is a highly appreciated mollusk with an important aquaculture production throughout the 20th century, in addition to playing an important role on coastal ecosystems. Overexploitation of natural beds, habitat degradation, introduction of non-native species and epidemic outbreaks have severely affected this important resource, particularly, the protozoan parasite Bonamia ostreae, which is the main concern affecting its
production and conservation. In order to identify genomic regions and markers potentially associated with bonamiosis resistance, six oyster beds distributed throughout the European Atlantic coast were sampled. Three of them have been exposed to this parasite since the early
1980’s and showed some degree of innate resistance (long-term affected group, LTA), while the other three were free of B. ostreae at least until sampling date (naïve group, NV). A total of 14,065 SNPs were analyzed, including 37 markers from candidate genes and 14,028 from a medium density SNP array. Gene diversity was similar between LTA and NV groups
suggesting no genetic erosion due to long term exposure to the parasite, and three population clusters were detected using the whole dataset. Tests for divergent selection between NV and LTA groups detected the presence of a very consistent set of 22 markers, located within a putative single genomic region, which suggests the presence of a major quantitative trait
locus associated with B. ostreae resistance. Moreover, 324 outlier loci associated with factors

Original languageEnglish
JournalEvolutionary Applications
Early online date19 Jun 2019
DOIs
Publication statusE-pub ahead of print - 19 Jun 2019

Keywords

  • Bonamia ostreae
  • candidate genes
  • disease resistance
  • divergent resistance
  • divergent selection
  • genetic traceability
  • ostrea edulis
  • SNP array

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