Silver nanoparticles promote the emergence of heterogeneic human neutrophil sub-populations

Jenny Fraser; Sadie Kemp; Lesley Young; Mark D Ross; Morag Prach; Gary R Hutchison; Eva Malone

doi:10.1038/s41598-018-25854-2

Silver nanoparticles promote the emergence of heterogeneic human neutrophil sub-populations

Jenny Fraser, Sadie Kemp, Lesley Young, Mark D Ross , Morag Prach, Gary R Hutchison, Eva Malone

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Neutrophil surveillance is central to nanoparticle clearance. Silver nanoparticles (AgNP) have numerous uses, however conficting evidence exists as to their impact on neutrophils and whether they trigger damaging infammation. Neutrophil’s importance in innate defence and regulating immune networks
mean it’s essential we understand AgNP’s impact on neutrophil function. Human neutrophil viability following AgNP or Ag Bulk treatment was analysed by fow cytometry and AnV/PI staining. Whilst AgNP exposure did not increase the total number of apoptotic neutrophils, the number of late apoptotic
neutrophils was increased, suggesting AgNP increase transit through apoptosis. Mature (CD16bright/ CD62Lbright), immature (CD16dim/CD62Lbright) and apoptotic (CD16dim/CD62Ldim) neutrophil populations were evident within isolated neutrophil preparations. AgNP exposure signifcantly reduced CD62L
staining of CD16bright/CD62Lbright neutrophils, and increased CD16 staining of CD16dim/CD62Lbright populations, suggesting AgNPs trigger neutrophil activation and maturation, respectively. AgNP exposure dramatically increased IL-8, yet not classical pro-infammatory cytokine release, suggesting
AgNP triggers neutrophil activation, without pro-infammation or damaging, necrotic cell death. For the frst time, we show AgNPs diferentially afect distinct sub-populations of circulating human neutrophils; activating mature neutrophils with the emergence of CD16bright/CD62Ldim neutrophils. This may stimulate particle clearance without harmful infammation, challenging previous assumptions that silver nanomaterials induce neutrophil toxicity and damaging infammatory responses.

Original language	English
Journal	Scientific Reports
Early online date	14 May 2018
DOIs	https://doi.org/10.1038/s41598-018-25854-2
Publication status	E-pub ahead of print - 14 May 2018

Access to Document

10.1038/s41598-018-25854-2

s41598-018-25854-2
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Final published version, 2.36 MBLicence: Creative Commons: Attribution (CC-BY)

Jenny Fraser
- Royal (Dick) School of Veterinary Studies - Lecturer/Senior Lecturer in Veterinary Biomedical Sciences
Person: Academic: Research Active

Cite this

@article{5d4887e9f2fb4bc49d5c29b09b0d27be,

title = "Silver nanoparticles promote the emergence of heterogeneic human neutrophil sub-populations",

abstract = "Neutrophil surveillance is central to nanoparticle clearance. Silver nanoparticles (AgNP) have numerous uses, however conficting evidence exists as to their impact on neutrophils and whether they trigger damaging infammation. Neutrophil{\textquoteright}s importance in innate defence and regulating immune networksmean it{\textquoteright}s essential we understand AgNP{\textquoteright}s impact on neutrophil function. Human neutrophil viability following AgNP or Ag Bulk treatment was analysed by fow cytometry and AnV/PI staining. Whilst AgNP exposure did not increase the total number of apoptotic neutrophils, the number of late apoptoticneutrophils was increased, suggesting AgNP increase transit through apoptosis. Mature (CD16bright/ CD62Lbright), immature (CD16dim/CD62Lbright) and apoptotic (CD16dim/CD62Ldim) neutrophil populations were evident within isolated neutrophil preparations. AgNP exposure signifcantly reduced CD62Lstaining of CD16bright/CD62Lbright neutrophils, and increased CD16 staining of CD16dim/CD62Lbright populations, suggesting AgNPs trigger neutrophil activation and maturation, respectively. AgNP exposure dramatically increased IL-8, yet not classical pro-infammatory cytokine release, suggestingAgNP triggers neutrophil activation, without pro-infammation or damaging, necrotic cell death. For the frst time, we show AgNPs diferentially afect distinct sub-populations of circulating human neutrophils; activating mature neutrophils with the emergence of CD16bright/CD62Ldim neutrophils. This may stimulate particle clearance without harmful infammation, challenging previous assumptions that silver nanomaterials induce neutrophil toxicity and damaging infammatory responses.",

author = "Jenny Fraser and Sadie Kemp and Lesley Young and Ross, {Mark D} and Morag Prach and Hutchison, {Gary R} and Eva Malone",

year = "2018",

month = may,

day = "14",

doi = "10.1038/s41598-018-25854-2",

language = "English",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Silver nanoparticles promote the emergence of heterogeneic human neutrophil sub-populations

AU - Fraser, Jenny

AU - Kemp, Sadie

AU - Young, Lesley

AU - Ross , Mark D

AU - Prach, Morag

AU - Hutchison, Gary R

AU - Malone, Eva

PY - 2018/5/14

Y1 - 2018/5/14

N2 - Neutrophil surveillance is central to nanoparticle clearance. Silver nanoparticles (AgNP) have numerous uses, however conficting evidence exists as to their impact on neutrophils and whether they trigger damaging infammation. Neutrophil’s importance in innate defence and regulating immune networksmean it’s essential we understand AgNP’s impact on neutrophil function. Human neutrophil viability following AgNP or Ag Bulk treatment was analysed by fow cytometry and AnV/PI staining. Whilst AgNP exposure did not increase the total number of apoptotic neutrophils, the number of late apoptoticneutrophils was increased, suggesting AgNP increase transit through apoptosis. Mature (CD16bright/ CD62Lbright), immature (CD16dim/CD62Lbright) and apoptotic (CD16dim/CD62Ldim) neutrophil populations were evident within isolated neutrophil preparations. AgNP exposure signifcantly reduced CD62Lstaining of CD16bright/CD62Lbright neutrophils, and increased CD16 staining of CD16dim/CD62Lbright populations, suggesting AgNPs trigger neutrophil activation and maturation, respectively. AgNP exposure dramatically increased IL-8, yet not classical pro-infammatory cytokine release, suggestingAgNP triggers neutrophil activation, without pro-infammation or damaging, necrotic cell death. For the frst time, we show AgNPs diferentially afect distinct sub-populations of circulating human neutrophils; activating mature neutrophils with the emergence of CD16bright/CD62Ldim neutrophils. This may stimulate particle clearance without harmful infammation, challenging previous assumptions that silver nanomaterials induce neutrophil toxicity and damaging infammatory responses.

AB - Neutrophil surveillance is central to nanoparticle clearance. Silver nanoparticles (AgNP) have numerous uses, however conficting evidence exists as to their impact on neutrophils and whether they trigger damaging infammation. Neutrophil’s importance in innate defence and regulating immune networksmean it’s essential we understand AgNP’s impact on neutrophil function. Human neutrophil viability following AgNP or Ag Bulk treatment was analysed by fow cytometry and AnV/PI staining. Whilst AgNP exposure did not increase the total number of apoptotic neutrophils, the number of late apoptoticneutrophils was increased, suggesting AgNP increase transit through apoptosis. Mature (CD16bright/ CD62Lbright), immature (CD16dim/CD62Lbright) and apoptotic (CD16dim/CD62Ldim) neutrophil populations were evident within isolated neutrophil preparations. AgNP exposure signifcantly reduced CD62Lstaining of CD16bright/CD62Lbright neutrophils, and increased CD16 staining of CD16dim/CD62Lbright populations, suggesting AgNPs trigger neutrophil activation and maturation, respectively. AgNP exposure dramatically increased IL-8, yet not classical pro-infammatory cytokine release, suggestingAgNP triggers neutrophil activation, without pro-infammation or damaging, necrotic cell death. For the frst time, we show AgNPs diferentially afect distinct sub-populations of circulating human neutrophils; activating mature neutrophils with the emergence of CD16bright/CD62Ldim neutrophils. This may stimulate particle clearance without harmful infammation, challenging previous assumptions that silver nanomaterials induce neutrophil toxicity and damaging infammatory responses.

U2 - 10.1038/s41598-018-25854-2

DO - 10.1038/s41598-018-25854-2

M3 - Article

SN - 2045-2322

JO - Scientific Reports

JF - Scientific Reports

ER -

Silver nanoparticles promote the emergence of heterogeneic human neutrophil sub-populations

Abstract / Description of output

Access to Document

Fingerprint

Profiles

Jenny Fraser

Cite this