Simultaneous Detection of Drug-Induced Liver Injury Protein and microRNA Biomarkers Using Dynamic Chemical Labelling on a Luminex MAGPIX System

Antonio Marín-Romero; Mavys Tabraue-Chávez; Barbara López-Longarela; Mario A. Fara; Rosario M. Sánchez-Martín; James W Dear; Hugh Ilyine; Juan J.  Díaz-Mochón; Salvatore Pernagallo

doi:10.3390/analytica2040013

Simultaneous Detection of Drug-Induced Liver Injury Protein and microRNA Biomarkers Using Dynamic Chemical Labelling on a Luminex MAGPIX System

Antonio Marín-Romero, Mavys Tabraue-Chávez, Barbara López-Longarela, Mario A. Fara, Rosario M. Sánchez-Martín, James W Dear, Hugh Ilyine, Juan J. Díaz-Mochón^*, Salvatore Pernagallo^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Drug-Induced Liver Injury (DILI) is a potentially fatal adverse event and a leading cause
for pre- and post-marketing drug withdrawal. Several multinational DILI initiatives have now rec-
ommended a panel of protein and microRNA (miRNA) biomarkers that can detect early liver injury
and inform about mechanistic basis. This manuscript describes the development of seqCOMBO, a
unique combo-multiplexed assay which combines the dynamic chemical labelling approach and an
antibody dependant method on Luminex MAGPIX system. SeqCOMBO enables a versatile multi-
plexing platform to perform qualitative and quantitative analysis of proteins and miRNAs in patient
serum samples simultaneously. To the best of our knowledge, this is the first method to profile
protein and miRNA biomarkers to diagnose DILI in a single step assay.

Original language	English
Journal	Analytica
DOIs	https://doi.org/10.3390/analytica2040013
Publication status	Published - 3 Oct 2021

Keywords / Materials (for Non-textual outputs)

dynamic chemical labelling (DCL)
drug-induced liver injury (DILI)
miRNA-122
Luminex MAGPIX
Liquid Biopsy
antibody-dependant method

Access to Document

10.3390/analytica2040013

manuscript.v6Accepted author manuscript, 278 KBLicence: Creative Commons: Attribution (CC-BY)

Cite this

@article{2f6a4724bfc8462db779aa7f9cff7101,

title = "Simultaneous Detection of Drug-Induced Liver Injury Protein and microRNA Biomarkers Using Dynamic Chemical Labelling on a Luminex MAGPIX System",

abstract = "Drug-Induced Liver Injury (DILI) is a potentially fatal adverse event and a leading cause for pre- and post-marketing drug withdrawal. Several multinational DILI initiatives have now rec- ommended a panel of protein and microRNA (miRNA) biomarkers that can detect early liver injury and inform about mechanistic basis. This manuscript describes the development of seqCOMBO, a unique combo-multiplexed assay which combines the dynamic chemical labelling approach and an antibody dependant method on Luminex MAGPIX system. SeqCOMBO enables a versatile multi- plexing platform to perform qualitative and quantitative analysis of proteins and miRNAs in patient serum samples simultaneously. To the best of our knowledge, this is the first method to profile protein and miRNA biomarkers to diagnose DILI in a single step assay.",

keywords = "dynamic chemical labelling (DCL), drug-induced liver injury (DILI), miRNA-122, Luminex MAGPIX, Liquid Biopsy, antibody-dependant method",

author = "Antonio Mar{\'i}n-Romero and Mavys Tabraue-Ch{\'a}vez and Barbara L{\'o}pez-Longarela and Fara, {Mario A.} and S{\'a}nchez-Mart{\'i}n, {Rosario M.} and Dear, {James W} and Hugh Ilyine and D{\'i}az-Moch{\'o}n, {Juan J.} and Salvatore Pernagallo",

year = "2021",

month = oct,

day = "3",

doi = "10.3390/analytica2040013",

language = "English",

journal = "Analytica",

issn = "2673-4532",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

}

TY - JOUR

T1 - Simultaneous Detection of Drug-Induced Liver Injury Protein and microRNA Biomarkers Using Dynamic Chemical Labelling on a Luminex MAGPIX System

AU - Marín-Romero, Antonio

AU - Tabraue-Chávez, Mavys

AU - López-Longarela, Barbara

AU - Fara, Mario A.

AU - Sánchez-Martín, Rosario M.

AU - Dear, James W

AU - Ilyine, Hugh

AU - Díaz-Mochón, Juan J.

AU - Pernagallo, Salvatore

PY - 2021/10/3

Y1 - 2021/10/3

N2 - Drug-Induced Liver Injury (DILI) is a potentially fatal adverse event and a leading cause for pre- and post-marketing drug withdrawal. Several multinational DILI initiatives have now rec- ommended a panel of protein and microRNA (miRNA) biomarkers that can detect early liver injury and inform about mechanistic basis. This manuscript describes the development of seqCOMBO, a unique combo-multiplexed assay which combines the dynamic chemical labelling approach and an antibody dependant method on Luminex MAGPIX system. SeqCOMBO enables a versatile multi- plexing platform to perform qualitative and quantitative analysis of proteins and miRNAs in patient serum samples simultaneously. To the best of our knowledge, this is the first method to profile protein and miRNA biomarkers to diagnose DILI in a single step assay.

AB - Drug-Induced Liver Injury (DILI) is a potentially fatal adverse event and a leading cause for pre- and post-marketing drug withdrawal. Several multinational DILI initiatives have now rec- ommended a panel of protein and microRNA (miRNA) biomarkers that can detect early liver injury and inform about mechanistic basis. This manuscript describes the development of seqCOMBO, a unique combo-multiplexed assay which combines the dynamic chemical labelling approach and an antibody dependant method on Luminex MAGPIX system. SeqCOMBO enables a versatile multi- plexing platform to perform qualitative and quantitative analysis of proteins and miRNAs in patient serum samples simultaneously. To the best of our knowledge, this is the first method to profile protein and miRNA biomarkers to diagnose DILI in a single step assay.

KW - dynamic chemical labelling (DCL)

KW - drug-induced liver injury (DILI)

KW - miRNA-122

KW - Luminex MAGPIX

KW - Liquid Biopsy

KW - antibody-dependant method

U2 - 10.3390/analytica2040013

DO - 10.3390/analytica2040013

M3 - Article

SN - 2673-4532

JO - Analytica

JF - Analytica

ER -

Simultaneous Detection of Drug-Induced Liver Injury Protein and microRNA Biomarkers Using Dynamic Chemical Labelling on a Luminex MAGPIX System

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

Access to Document

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