Simultaneous Spectral Temporal Modelling for a Time-Resolved Fluorescence Emission Spectrum

Alexandra Adams; Andras Kufcsak; Katjana Ehrlich; Kevin Dhaliwal; Sohan Seth

doi:10.1109/TBME.2023.3244664

Simultaneous Spectral Temporal Modelling for a Time-Resolved Fluorescence Emission Spectrum

Alexandra Adams, Andras Kufcsak, Katjana Ehrlich, Kevin Dhaliwal, Sohan Seth

Research output: Contribution to journal › Article › peer-review

Abstract

Innovations in complementary metal-oxide semiconductor (CMOS) single-photon avalanche diode (SPAD) technology has featured in the development of nextgeneration instruments for point-based time-resolved fluorescence spectroscopy (TRFS). These instruments provide hundreds of spectral channels, allowing the collection of fluorescence intensity and fluorescence lifetime information over a broad spectral range at a high spectral and temporal resolution. We present Multichannel Fluorescence Lifetime Estimation, MuFLE, an efficient computational approach to exploit the unique multi-channel spectroscopy data with an emphasis on simultaneous estimation of the emission spectra, and the respective spectral fluorescence lifetimes. In addition, we show that this approach can estimate the individual spectral characteristics of fluorophores from a mixed sample.

Original language	English
Pages (from-to)	2395-2403
Journal	IEEE Transactions on Biomedical Engineering
Volume	70
Issue number	8
DOIs	https://doi.org/10.1109/TBME.2023.3244664
Publication status	Published - 19 Jul 2023

Keywords / Materials (for Non-textual outputs)

Fluorescence lifetime
time-resolved fluorescence spectroscopy
B-splines
non-linear least squares

Access to Document

10.1109/TBME.2023.3244664Licence: All Rights Reserved

Simultaneous Spectral Temporal_ADAMS_DOA0102023_AFV_CC-BYAccepted author manuscript, 11.4 MBLicence: Creative Commons: Attribution (CC-BY)

https://ieeexplore.ieee.org/document/10068267Licence: All Rights Reserved

Cite this

@article{30ea56c245ee4505a86d4fb5e43e7a40,

title = "Simultaneous Spectral Temporal Modelling for a Time-Resolved Fluorescence Emission Spectrum",

abstract = "Innovations in complementary metal-oxide semiconductor (CMOS) single-photon avalanche diode (SPAD) technology has featured in the development of nextgeneration instruments for point-based time-resolved fluorescence spectroscopy (TRFS). These instruments provide hundreds of spectral channels, allowing the collection of fluorescence intensity and fluorescence lifetime information over a broad spectral range at a high spectral and temporal resolution. We present Multichannel Fluorescence Lifetime Estimation, MuFLE, an efficient computational approach to exploit the unique multi-channel spectroscopy data with an emphasis on simultaneous estimation of the emission spectra, and the respective spectral fluorescence lifetimes. In addition, we show that this approach can estimate the individual spectral characteristics of fluorophores from a mixed sample.",

keywords = "Fluorescence lifetime, time-resolved fluorescence spectroscopy, B-splines, non-linear least squares",

author = "Alexandra Adams and Andras Kufcsak and Katjana Ehrlich and Kevin Dhaliwal and Sohan Seth",

note = "A. A. was supported by the Medical Research Council [grant number MR/N13 166/1]. K. E. was supported by EP/V6185/1",

year = "2023",

month = jul,

day = "19",

doi = "10.1109/TBME.2023.3244664",

language = "English",

volume = "70",

pages = "2395--2403",

journal = "IEEE Transactions on Biomedical Engineering",

issn = "0018-9294",

publisher = "IEEE Computer Society",

number = "8",

}

TY - JOUR

T1 - Simultaneous Spectral Temporal Modelling for a Time-Resolved Fluorescence Emission Spectrum

AU - Adams, Alexandra

AU - Kufcsak, Andras

AU - Ehrlich, Katjana

AU - Dhaliwal, Kevin

AU - Seth, Sohan

N1 - A. A. was supported by the Medical Research Council [grant number MR/N13 166/1]. K. E. was supported by EP/V6185/1

PY - 2023/7/19

Y1 - 2023/7/19

N2 - Innovations in complementary metal-oxide semiconductor (CMOS) single-photon avalanche diode (SPAD) technology has featured in the development of nextgeneration instruments for point-based time-resolved fluorescence spectroscopy (TRFS). These instruments provide hundreds of spectral channels, allowing the collection of fluorescence intensity and fluorescence lifetime information over a broad spectral range at a high spectral and temporal resolution. We present Multichannel Fluorescence Lifetime Estimation, MuFLE, an efficient computational approach to exploit the unique multi-channel spectroscopy data with an emphasis on simultaneous estimation of the emission spectra, and the respective spectral fluorescence lifetimes. In addition, we show that this approach can estimate the individual spectral characteristics of fluorophores from a mixed sample.

AB - Innovations in complementary metal-oxide semiconductor (CMOS) single-photon avalanche diode (SPAD) technology has featured in the development of nextgeneration instruments for point-based time-resolved fluorescence spectroscopy (TRFS). These instruments provide hundreds of spectral channels, allowing the collection of fluorescence intensity and fluorescence lifetime information over a broad spectral range at a high spectral and temporal resolution. We present Multichannel Fluorescence Lifetime Estimation, MuFLE, an efficient computational approach to exploit the unique multi-channel spectroscopy data with an emphasis on simultaneous estimation of the emission spectra, and the respective spectral fluorescence lifetimes. In addition, we show that this approach can estimate the individual spectral characteristics of fluorophores from a mixed sample.

KW - Fluorescence lifetime

KW - time-resolved fluorescence spectroscopy

KW - B-splines

KW - non-linear least squares

U2 - 10.1109/TBME.2023.3244664

DO - 10.1109/TBME.2023.3244664

M3 - Article

SN - 0018-9294

VL - 70

SP - 2395

EP - 2403

JO - IEEE Transactions on Biomedical Engineering

JF - IEEE Transactions on Biomedical Engineering

IS - 8

ER -

Simultaneous Spectral Temporal Modelling for a Time-Resolved Fluorescence Emission Spectrum

Abstract

Keywords / Materials (for Non-textual outputs)

Access to Document

Fingerprint

Cite this