TY - JOUR
T1 - Single Bead Labeling Method for Combining Confocal Fluorescence On-Bead Screening and Solution Validation of Tagged One-Bead One-Compound Libraries
AU - Hintersteiner, Martin
AU - Kimmerlin, Thierry
AU - Kalthoff, Frank
AU - Stoeckli, Markus
AU - Garavel, Geraldine
AU - Seifert, Jan-Marcus
AU - Meisner, Nicole-Claudia
AU - Uhl, Volker
AU - Buehler, Christof
AU - Weidemann, Thomas
AU - Auer, Manfred
PY - 2009/7/31
Y1 - 2009/7/31
N2 - Screening of one-bead one-compound libraries by incubating beads with fluorescently labeled target protein requires isolation and structure elucidation of a large number of primary hit beads. However, the potency of the identified ligands is only revealed after time consuming and expensive larger scale resynthesis and testing in solution. Often, many of the resynthesized compounds turn out to be weak target binders in solution due to large differences between surface and solution binding affinities. For an industry style high-throughput screening (HTS) process a high false positive rate is detrimental. We have therefore combined single bead and single molecule/single cell techniques into an integrated HTS process in which the picomole amount of substance contained on one isolated hit bead is sufficient for quality control, structure determination, and precise affinity determination to the target protein in solution.
AB - Screening of one-bead one-compound libraries by incubating beads with fluorescently labeled target protein requires isolation and structure elucidation of a large number of primary hit beads. However, the potency of the identified ligands is only revealed after time consuming and expensive larger scale resynthesis and testing in solution. Often, many of the resynthesized compounds turn out to be weak target binders in solution due to large differences between surface and solution binding affinities. For an industry style high-throughput screening (HTS) process a high false positive rate is detrimental. We have therefore combined single bead and single molecule/single cell techniques into an integrated HTS process in which the picomole amount of substance contained on one isolated hit bead is sufficient for quality control, structure determination, and precise affinity determination to the target protein in solution.
U2 - 10.1016/j.chembiol.2009.06.011
DO - 10.1016/j.chembiol.2009.06.011
M3 - Article
SN - 1074-5521
VL - 16
SP - 724
EP - 735
JO - Chemistry and Biology
JF - Chemistry and Biology
IS - 7
ER -