Single-cell proteomics defines the cellular heterogeneity of localized prostate cancer

Laura De Vargas Roditi; Andrea Jacobs; Jan H. Rueschoff; Pete Bankhead; Stéphane Chevrier; Hartland W. Jackson; Thomas Hermanns; Christian D. Fankhauser; Cedric Poyet; Felix Chun; Niels J. Rupp; Alexandra Tschaebunin; Bernd Bodenmiller; Peter J. Wild

doi:10.1016/j.xcrm.2022.100604

Single-cell proteomics defines the cellular heterogeneity of localized prostate cancer

Laura De Vargas Roditi, Andrea Jacobs, Jan H. Rueschoff, Pete Bankhead, Stéphane Chevrier, Hartland W. Jackson, Thomas Hermanns, Christian D. Fankhauser, Cedric Poyet, Felix Chun, Niels J. Rupp, Alexandra Tschaebunin, Bernd Bodenmiller, Peter J. Wild

Research output: Contribution to journal › Article › peer-review

Original language	English
Pages (from-to)	100604
Journal	Cell Reports Medicine
Volume	3
Issue number	4
DOIs	https://doi.org/10.1016/j.xcrm.2022.100604
Publication status	Published - 19 Apr 2022

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De Vargas Roditi, L., Jacobs, A., Rueschoff, J. H., Bankhead, P., Chevrier, S., Jackson, H. W., Hermanns, T., Fankhauser, C. D., Poyet, C., Chun, F., Rupp, N. J., Tschaebunin, A., Bodenmiller, B., & Wild, P. J. (2022). Single-cell proteomics defines the cellular heterogeneity of localized prostate cancer. Cell Reports Medicine, 3(4), 100604. https://doi.org/10.1016/j.xcrm.2022.100604

@article{04d3db972d6e416d86dc576d95b3491b,

title = "Single-cell proteomics defines the cellular heterogeneity of localized prostate cancer",

author = "{De Vargas Roditi}, Laura and Andrea Jacobs and Rueschoff, {Jan H.} and Pete Bankhead and St{\'e}phane Chevrier and Jackson, {Hartland W.} and Thomas Hermanns and Fankhauser, {Christian D.} and Cedric Poyet and Felix Chun and Rupp, {Niels J.} and Alexandra Tschaebunin and Bernd Bodenmiller and Wild, {Peter J.}",

note = "Funding Information: The research was supported by a European Research Council PrECISE project from the European Union Horizon 2020 research and innovation program under grant agreement No. 668858 . We thank the Wild and Bodenmiller lab members as well as Dr. Andreas Moor for useful discussions and valuable feedback. Funding Information: The research was supported by a European Research Council PrECISE project from the European Union Horizon 2020 research and innovation program under grant agreement No. 668858. We thank the Wild and Bodenmiller lab members as well as Dr. Andreas Moor for useful discussions and valuable feedback. L.D.V.R. B.B. and P.W. conceived the study. P.W. B.B. A.J. and L.D.V.R designed the antibody panel and A.J. performed all antibody validation and data acquisition experiments. L.D.V.R. designed and developed Franken and performed data analysis. L.D.V.R and S.C tested algorithm in multiple datasets. T.H. C.P. and C.D.F. provided clinical samples. L.D.V.R. and P.W. developed TMA. P.W. P.B. J.H.R. A.T. and L.D.V.R. performed the TMA IHC image analysis. L.D.V.R. B.B. and P.W. performed biological analysis and interpretation with input from J.H.R and H.W.J.; L.D.V.R, B.B. and P.W. wrote the manuscript with input from F.C. H.W.J, S.C. and C.D.F. P.J.W. has received consulting fees and honoraria (institutional/personal) for lectures by Bayer, Janssen-Cilag, Novartis, Roche, MSD, Astellas Pharma, Bristol-Myers Squibb, Thermo Fisher Scientific, Molecular Health, Sophia Genetics, Qiagen, and Astra Zeneca. L.D.V.R. is an employee at Monte Rosa Therapeutics. All other authors have declared no competing interests. Publisher Copyright: {\textcopyright} 2022 The Author(s)",

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doi = "10.1016/j.xcrm.2022.100604",

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De Vargas Roditi, L, Jacobs, A, Rueschoff, JH, Bankhead, P, Chevrier, S, Jackson, HW, Hermanns, T, Fankhauser, CD, Poyet, C, Chun, F, Rupp, NJ, Tschaebunin, A, Bodenmiller, B & Wild, PJ 2022, 'Single-cell proteomics defines the cellular heterogeneity of localized prostate cancer', Cell Reports Medicine, vol. 3, no. 4, pp. 100604. https://doi.org/10.1016/j.xcrm.2022.100604

TY - JOUR

T1 - Single-cell proteomics defines the cellular heterogeneity of localized prostate cancer

AU - De Vargas Roditi, Laura

AU - Jacobs, Andrea

AU - Rueschoff, Jan H.

AU - Bankhead, Pete

AU - Chevrier, Stéphane

AU - Jackson, Hartland W.

AU - Hermanns, Thomas

AU - Fankhauser, Christian D.

AU - Poyet, Cedric

AU - Chun, Felix

AU - Rupp, Niels J.

AU - Tschaebunin, Alexandra

AU - Bodenmiller, Bernd

AU - Wild, Peter J.

N1 - Funding Information: The research was supported by a European Research Council PrECISE project from the European Union Horizon 2020 research and innovation program under grant agreement No. 668858 . We thank the Wild and Bodenmiller lab members as well as Dr. Andreas Moor for useful discussions and valuable feedback. Funding Information: The research was supported by a European Research Council PrECISE project from the European Union Horizon 2020 research and innovation program under grant agreement No. 668858. We thank the Wild and Bodenmiller lab members as well as Dr. Andreas Moor for useful discussions and valuable feedback. L.D.V.R. B.B. and P.W. conceived the study. P.W. B.B. A.J. and L.D.V.R designed the antibody panel and A.J. performed all antibody validation and data acquisition experiments. L.D.V.R. designed and developed Franken and performed data analysis. L.D.V.R and S.C tested algorithm in multiple datasets. T.H. C.P. and C.D.F. provided clinical samples. L.D.V.R. and P.W. developed TMA. P.W. P.B. J.H.R. A.T. and L.D.V.R. performed the TMA IHC image analysis. L.D.V.R. B.B. and P.W. performed biological analysis and interpretation with input from J.H.R and H.W.J.; L.D.V.R, B.B. and P.W. wrote the manuscript with input from F.C. H.W.J, S.C. and C.D.F. P.J.W. has received consulting fees and honoraria (institutional/personal) for lectures by Bayer, Janssen-Cilag, Novartis, Roche, MSD, Astellas Pharma, Bristol-Myers Squibb, Thermo Fisher Scientific, Molecular Health, Sophia Genetics, Qiagen, and Astra Zeneca. L.D.V.R. is an employee at Monte Rosa Therapeutics. All other authors have declared no competing interests. Publisher Copyright: © 2022 The Author(s)

PY - 2022/4/19

Y1 - 2022/4/19

U2 - 10.1016/j.xcrm.2022.100604

DO - 10.1016/j.xcrm.2022.100604

M3 - Article

SN - 2666-3791

VL - 3

SP - 100604

JO - Cell Reports Medicine

JF - Cell Reports Medicine

IS - 4

ER -