Single-Channel Recording of Glycine Receptors in Human Embryonic Kidney (HEK) Cells

Andrew J R Plested, Jelena Baranovic

Research output: Contribution to journalArticlepeer-review

Abstract

This protocol describes how to record the single-channel activity of recombinant homomeric glycine receptors expressed in human embryonic kidney (HEK) cells. Cell-attached recordings readily reveal the large conductance (90 pS) and distinctive clusters of activations at high glycine concentration. This method for obtaining equilibrium recordings can be adapted to any ion channel receptor. The necessary extensions to outside-out patch for nonequilibrium recordings are also described, as are basic analyses of channel properties and activity.

Original languageEnglish
Pages (from-to)693-698
Number of pages6
JournalCold Spring Harbor protocols
Volume2016
Issue number8
DOIs
Publication statusPublished - 1 Aug 2016

Keywords

  • Cells, Cultured
  • Epithelial Cells/physiology
  • Gene Expression
  • Humans
  • Kidney
  • Patch-Clamp Techniques/methods
  • Receptors, Glycine/analysis
  • Recombinant Proteins/analysis

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