This protocol describes how to record the single-channel activity of recombinant homomeric glycine receptors expressed in human embryonic kidney (HEK) cells. Cell-attached recordings readily reveal the large conductance (90 pS) and distinctive clusters of activations at high glycine concentration. This method for obtaining equilibrium recordings can be adapted to any ion channel receptor. The necessary extensions to outside-out patch for nonequilibrium recordings are also described, as are basic analyses of channel properties and activity.
- Cells, Cultured
- Epithelial Cells/physiology
- Gene Expression
- Patch-Clamp Techniques/methods
- Receptors, Glycine/analysis
- Recombinant Proteins/analysis