Single isolated vesicles in microfluidic traps to study membrane protein kinetics

Tom Robinson, Phillip Kuhn, Klaus Eyer, Petra S. Dittrich*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a microfluidic platform that is able to trap and isolate single giant unilamellar vesicles (GUVs) in parallel. To demonstrate this, the membrane pore protein α-hemolysin (αHL) was added to GUVs and the kinetics of small molecule release was recorded.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1519-1521
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 28 Oct 20121 Nov 2012

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Conference

Conference16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Country/TerritoryJapan
CityOkinawa
Period28/10/121/11/12

Keywords / Materials (for Non-textual outputs)

  • GUVs
  • Lipids
  • Membrane proteins
  • Membranes
  • Transport
  • Vesicles
  • α-hemolysin

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