Abstract
The ability to routinely detect fluorescent nucleobase analogues at the single-molecule level would create a wealth of opportunities to study nucleic acids. We report the multiphoton-induced fluorescence and single-molecule detection of a dimethylamine-substituted extended-6-aza-uridine (DMAthaU). We show that DMAthaU can exist in a highly fluorescent form, emitting strongly in the visible region (470–560 nm). Using pulse-shaped broadband Ti:sapphire laser excitation, DMAthaU undergoes two-photon (2P) absorption at low excitation powers, switching to three-photon (3P) absorption at high incident intensity. The assignment of a 3P process is supported by cubic response calculations. Under both 2P and 3P excitation, the single-molecule brightness was over an order of magnitude higher than reported previously for any fluorescent base analogue, which facilitated the first single-molecule detection of an emissive nucleoside with multiphoton excitation.
Original language | English |
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Pages (from-to) | 5008-5012 |
Journal | The Journal of Physical Chemistry Letters |
Early online date | 9 Aug 2019 |
DOIs | |
Publication status | E-pub ahead of print - 9 Aug 2019 |
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Anita Jones
- School of Chemistry - Personal Chair in Molecular Photophysics
- EaStCHEM
Person: Academic: Research Active