Projects per year
Direct microscopic fluorescence imaging of single molecules can provide a wealth of mechanistic information, but up to now, it has not been possible under high pressure conditions, due to limitations in microscope pressure cell design. We describe a pressure cell window design that makes it possible to image directly single molecules at high hydrostatic pressure. We demonstrate our design by imaging single molecules of Alexa Fluor 647 dye bound to DNA, at 120 and 210 bar, and following their fluorescence photodynamics. We further show that the failure pressure of this type of pressure cell window can be in excess of 1 kbar. (C) 2013 AIP Publishing LLC [http://dx.doi.org/10.1063/1.4802202]
|Number of pages||4|
|Journal||Applied Physics Letters|
|Publication status||Published - 15 Apr 2013|
- OPTICAL CHAMBER SYSTEM
- LIVING CELLS
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- 1 Finished
Design Principles for New Soft Materials
Cates, M., Allen, R., Clegg, P., Evans, M., MacPhee, C., Marenduzzo, D. & Poon, W.
7/12/11 → 6/06/17