Single-molecule imaging at high hydrostatic pressure

Hugh Vass; S. Lucas Black; Cristina Flors; Diarmuid Lloyd; F. Bruce Ward; Rosalind J. Allen

doi:10.1063/1.4802202

Single-molecule imaging at high hydrostatic pressure

Hugh Vass^*, S. Lucas Black, Cristina Flors, Diarmuid Lloyd, F. Bruce Ward, Rosalind J. Allen

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Direct microscopic fluorescence imaging of single molecules can provide a wealth of mechanistic information, but up to now, it has not been possible under high pressure conditions, due to limitations in microscope pressure cell design. We describe a pressure cell window design that makes it possible to image directly single molecules at high hydrostatic pressure. We demonstrate our design by imaging single molecules of Alexa Fluor 647 dye bound to DNA, at 120 and 210 bar, and following their fluorescence photodynamics. We further show that the failure pressure of this type of pressure cell window can be in excess of 1 kbar. (C) 2013 AIP Publishing LLC [http://dx.doi.org/10.1063/1.4802202]

Original language	English
Article number	154103
Number of pages	4
Journal	Applied Physics Letters
Volume	102
Issue number	15
DOIs	https://doi.org/10.1063/1.4802202
Publication status	Published - 15 Apr 2013

Keywords

OPTICAL CHAMBER SYSTEM
LIVING CELLS
MICROSCOPY
TEMPERATURE
PROTEINS
SPECTROSCOPY
TRANSCRIPTION
BIOMOLECULES
MECHANISM
KINETICS

Access to Document

10.1063/1.4802202

Design Principles for New Soft Materials
Cates, M., Allen, R., Clegg, P., Evans, M., MacPhee, C., Marenduzzo, D. & Poon, W.
EPSRC
7/12/11 → 6/06/17
Project: Research

Cite this

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title = "Single-molecule imaging at high hydrostatic pressure",

abstract = "Direct microscopic fluorescence imaging of single molecules can provide a wealth of mechanistic information, but up to now, it has not been possible under high pressure conditions, due to limitations in microscope pressure cell design. We describe a pressure cell window design that makes it possible to image directly single molecules at high hydrostatic pressure. We demonstrate our design by imaging single molecules of Alexa Fluor 647 dye bound to DNA, at 120 and 210 bar, and following their fluorescence photodynamics. We further show that the failure pressure of this type of pressure cell window can be in excess of 1 kbar. (C) 2013 AIP Publishing LLC [http://dx.doi.org/10.1063/1.4802202]",

keywords = "OPTICAL CHAMBER SYSTEM, LIVING CELLS, MICROSCOPY, TEMPERATURE, PROTEINS, SPECTROSCOPY, TRANSCRIPTION, BIOMOLECULES, MECHANISM, KINETICS",

author = "Hugh Vass and Black, {S. Lucas} and Cristina Flors and Diarmuid Lloyd and Ward, {F. Bruce} and Allen, {Rosalind J.}",

year = "2013",

month = apr,

day = "15",

doi = "10.1063/1.4802202",

language = "English",

volume = "102",

journal = "Applied Physics Letters",

issn = "0003-6951",

publisher = "American Institute of Physics Publising LLC",

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TY - JOUR

T1 - Single-molecule imaging at high hydrostatic pressure

AU - Vass, Hugh

AU - Black, S. Lucas

AU - Flors, Cristina

AU - Lloyd, Diarmuid

AU - Ward, F. Bruce

AU - Allen, Rosalind J.

PY - 2013/4/15

Y1 - 2013/4/15

N2 - Direct microscopic fluorescence imaging of single molecules can provide a wealth of mechanistic information, but up to now, it has not been possible under high pressure conditions, due to limitations in microscope pressure cell design. We describe a pressure cell window design that makes it possible to image directly single molecules at high hydrostatic pressure. We demonstrate our design by imaging single molecules of Alexa Fluor 647 dye bound to DNA, at 120 and 210 bar, and following their fluorescence photodynamics. We further show that the failure pressure of this type of pressure cell window can be in excess of 1 kbar. (C) 2013 AIP Publishing LLC [http://dx.doi.org/10.1063/1.4802202]

AB - Direct microscopic fluorescence imaging of single molecules can provide a wealth of mechanistic information, but up to now, it has not been possible under high pressure conditions, due to limitations in microscope pressure cell design. We describe a pressure cell window design that makes it possible to image directly single molecules at high hydrostatic pressure. We demonstrate our design by imaging single molecules of Alexa Fluor 647 dye bound to DNA, at 120 and 210 bar, and following their fluorescence photodynamics. We further show that the failure pressure of this type of pressure cell window can be in excess of 1 kbar. (C) 2013 AIP Publishing LLC [http://dx.doi.org/10.1063/1.4802202]

KW - OPTICAL CHAMBER SYSTEM

KW - LIVING CELLS

KW - MICROSCOPY

KW - TEMPERATURE

KW - PROTEINS

KW - SPECTROSCOPY

KW - TRANSCRIPTION

KW - BIOMOLECULES

KW - MECHANISM

KW - KINETICS

U2 - 10.1063/1.4802202

DO - 10.1063/1.4802202

M3 - Article

VL - 102

JO - Applied Physics Letters

JF - Applied Physics Letters

SN - 0003-6951

IS - 15

M1 - 154103

ER -

Single-molecule imaging at high hydrostatic pressure

Abstract

Keywords

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Design Principles for New Soft Materials

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