Single molecule tracking and spectroscopy unveils molecular details in function and interactions of membrane receptors

Laura Marchetti, Fulvio Bonsignore, Rosy Amodeo, Chiara Schirripa Spagnolo, Aldo Moscardini, Francesco Gobbo, Antonino Cattaneo, Fabio Beltram, Stefano Luin*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract / Description of output

We exploited, optimized, and developed various microscopy techniques for analyzing living matter at cellular and molecular levels. In particular, we developed a toolbox for single particle tracking (SPT) of membrane receptors and their ligands, suitable also for relatively fast single-pass membrane receptors; this is based on chemical tagging of recombinant proteins, TIRF microscopy, and automatized analysis of single particle trajectories. We are now extending it to simultaneous visualization and analysis of two moieties. The superresolved localization of this technique allowed analyzing functions, interactions and stoichiometry of (pro)neurotrophin receptors p75NTR and TrkA and of their ligands in living cells, with particular attention on some of their existing or used mutants. The analysis also after treatments with ligands or drugs unraveled their mode of action in the first steps of sundry signaling pathways.

Original languageEnglish
Title of host publicationSingle Molecule Spectroscopy and Superresolution Imaging XIV
EditorsIngo Gregor, Felix Koberling, Rainer Erdmann
PublisherSPIE
ISBN (Electronic)9781510641358
DOIs
Publication statusPublished - 5 Mar 2021
EventSingle Molecule Spectroscopy and Superresolution Imaging XIV 2021 - Virtual, Online, United States
Duration: 6 Mar 202111 Mar 2021

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11650
ISSN (Print)1605-7422

Conference

ConferenceSingle Molecule Spectroscopy and Superresolution Imaging XIV 2021
Country/TerritoryUnited States
CityVirtual, Online
Period6/03/2111/03/21

Keywords / Materials (for Non-textual outputs)

  • Chemical tags
  • Living cells
  • Membrane dynamics
  • Neurotrophin receptors
  • Oligomeric state
  • Single molecule labelling
  • Single Particle Tracking
  • TIRF microscopy

Fingerprint

Dive into the research topics of 'Single molecule tracking and spectroscopy unveils molecular details in function and interactions of membrane receptors'. Together they form a unique fingerprint.

Cite this