@inproceedings{2ec8a4a8eeb942ae9ef878b46c1685f0,
title = "Single molecule tracking and spectroscopy unveils molecular details in function and interactions of membrane receptors",
abstract = "We exploited, optimized, and developed various microscopy techniques for analyzing living matter at cellular and molecular levels. In particular, we developed a toolbox for single particle tracking (SPT) of membrane receptors and their ligands, suitable also for relatively fast single-pass membrane receptors; this is based on chemical tagging of recombinant proteins, TIRF microscopy, and automatized analysis of single particle trajectories. We are now extending it to simultaneous visualization and analysis of two moieties. The superresolved localization of this technique allowed analyzing functions, interactions and stoichiometry of (pro)neurotrophin receptors p75NTR and TrkA and of their ligands in living cells, with particular attention on some of their existing or used mutants. The analysis also after treatments with ligands or drugs unraveled their mode of action in the first steps of sundry signaling pathways.",
keywords = "Chemical tags, Living cells, Membrane dynamics, Neurotrophin receptors, Oligomeric state, Single molecule labelling, Single Particle Tracking, TIRF microscopy",
author = "Laura Marchetti and Fulvio Bonsignore and Rosy Amodeo and Spagnolo, {Chiara Schirripa} and Aldo Moscardini and Francesco Gobbo and Antonino Cattaneo and Fabio Beltram and Stefano Luin",
note = "Publisher Copyright: {\textcopyright} 2021.; Single Molecule Spectroscopy and Superresolution Imaging XIV 2021 ; Conference date: 06-03-2021 Through 11-03-2021",
year = "2021",
month = mar,
day = "5",
doi = "10.1117/12.2578193",
language = "English",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Ingo Gregor and Felix Koberling and Rainer Erdmann",
booktitle = "Single Molecule Spectroscopy and Superresolution Imaging XIV",
address = "United States",
}