SINGLE-PARTICLE APPLICATIONS AT INTERMEDIATE RESOLUTION

Bettina Boettcher, Katharina Hipp

Research output: Chapter in Book/Report/Conference proceedingOther chapter contribution

Abstract

Electron microscopy together with single-particle image processing is an excellent method for structure determination of biological assemblies that exist in multiple identical copies. Typical assemblies contain several proteins and/or nucleic acids in a defined and reproducible arrangement. Coherent averaging of electron microscopic images of 5000-100,000 copies of these assemblies allows the determination of three-dimensional structures at ca. 1-3-nm resolution. At this intermediate resolution, it is possible to map individual subunits and thus to understand the architecture and quaternary structure of the assemblies. The intermediate resolution structural information gives a solid basis on which pseudo-atomic models of the assemblies can be modeled provided that high-resolution structures of smaller entities are known. The architecture of the assemblies, their pseudo-atomic models, and knowledge on their plasticity during function give a comprehensive understanding of large-scale structural dynamics of multicopy biological complexes. In this review, we will introduce the experimental pipeline and discuss selected examples.

Original languageEnglish
Title of host publicationADVANCES IN PROTEIN CHEMISTRY AND STRUCTURAL BIOLOGY: RECENT ADVANCES IN ELECTRON CRYOMICROSCOPY, PT A
Place of PublicationSAN DIEGO
PublisherELSEVIER ACADEMIC PRESS INC
Pages61-88
Number of pages28
DOIs
Publication statusPublished - 2010

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