Singlet oxygen photosensitisation by GFP mutants: oxygen accessibility to the chromophore

Ana Jimenez-Banzo, Xavier Ragas, Stefania Abbruzzetti, Cristiano Viappiani, Barbara Campanini, Cristina Flors, Santi Nonell

Research output: Contribution to journalArticlepeer-review

Abstract

Aiming at the rational development of genetically-encoded photosensitisers, the production of singlet oxygen has been assessed for a number of class-2 Green Fluorescent Protein (GFP) mutants by means of time-resolved near-infrared luminescence detection. The accessibility of molecular oxygen to the chromophore seems to play a major role in the ability of GFPs to photosensitise singlet oxygen and this can be modulated by introducing specific mutations such as replacement of His148 by a less bulky amino acid. GFPs are also good singlet oxygen quenchers, hence further developments in this area should also seek to eliminate those amino acids with the highest quenching ability, particularly those at the protein surface and in the vicinity of the chromophore.

Original languageEnglish
Pages (from-to)1336-1341
Number of pages6
JournalPhotochemical & Photobiological Sciences
Volume9
Issue number10
DOIs
Publication statusPublished - 2010

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