Sister kinetochores are mechanically fused during meiosis I in yeast

Krishna K. Sarangapani, Eris Duro, Yi Deng, Flavia De Lima Alves, Qiaozhen Ye, Kwaku N. Opoku, Steven Ceto, Juri Rappsilber, Kevin D. Corbett, Sue Biggins, Adèle L. Marston, Charles L. Asbury*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Production of healthy gametes requires a reductional meiosis I division in which replicated sister chromatids comigrate, rather than separate as in mitosis or meiosis II. Fusion of sister kinetochores during meiosis I may underlie sister chromatid comigration in diverse organisms, but direct evidence for such fusion has been lacking.We used laser trapping and quantitative fluorescence microscopy to study native kinetochore particles isolated from yeast. Meiosis I kinetochores formed stronger attachments and carried more microtubule-binding elements than kinetochores isolated from cells in mitosis or meiosis II. The meiosis I-specific monopolin complex was both necessary and sufficient to drive these modifications. Thus, kinetochore fusion directs sister chromatid comigration, a conserved feature of meiosis that is fundamental to Mendelian inheritance.

Original languageEnglish
Pages (from-to)248-251
Number of pages4
Issue number6206
Publication statusPublished - 10 Oct 2014


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