Site-Specific Direct Labeling of Neurotrophins and Their Receptors: From Biochemistry to Advanced Imaging Applications

Francesco Gobbo, Fulvio Bonsignore, Rosy Amodeo, Antonino Cattaneo, Laura Marchetti

Research output: Contribution to journalArticlepeer-review

Abstract

We describe here a versatile methodological platform to achieve site-directed and stoichiometry-controlled labeling of neurotrophins and their receptors with various probes, ranging from biotin to small organic dyes. This labeling method works in vitro on purified neurotrophins as well as in a living cell context, where it achieves selective labeling of surface-exposed neurotrophin receptors. Here, we list all experimental details of our labeling protocols, along with examples of the wide range of applications in which these can be used.

Original languageEnglish
Pages (from-to)295-314
Number of pages20
JournalMethods in Molecular Biology
Volume1727
DOIs
Publication statusPublished - 2018
Externally publishedYes

Keywords

  • Animals
  • Biotin/chemistry
  • Cell Line
  • Fluorescent Dyes/chemistry
  • HEK293 Cells
  • Humans
  • Mice
  • Nerve Growth Factors/chemistry
  • Receptors, Nerve Growth Factor/chemistry
  • Staining and Labeling

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