Abstract
We describe here a versatile methodological platform to achieve site-directed and stoichiometry-controlled labeling of neurotrophins and their receptors with various probes, ranging from biotin to small organic dyes. This labeling method works in vitro on purified neurotrophins as well as in a living cell context, where it achieves selective labeling of surface-exposed neurotrophin receptors. Here, we list all experimental details of our labeling protocols, along with examples of the wide range of applications in which these can be used.
Original language | English |
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Pages (from-to) | 295-314 |
Number of pages | 20 |
Journal | Methods in Molecular Biology |
Volume | 1727 |
DOIs | |
Publication status | Published - 2018 |
Externally published | Yes |
Keywords / Materials (for Non-textual outputs)
- Animals
- Biotin/chemistry
- Cell Line
- Fluorescent Dyes/chemistry
- HEK293 Cells
- Humans
- Mice
- Nerve Growth Factors/chemistry
- Receptors, Nerve Growth Factor/chemistry
- Staining and Labeling