We describe here a versatile methodological platform to achieve site-directed and stoichiometry-controlled labeling of neurotrophins and their receptors with various probes, ranging from biotin to small organic dyes. This labeling method works in vitro on purified neurotrophins as well as in a living cell context, where it achieves selective labeling of surface-exposed neurotrophin receptors. Here, we list all experimental details of our labeling protocols, along with examples of the wide range of applications in which these can be used.
- Cell Line
- Fluorescent Dyes/chemistry
- HEK293 Cells
- Nerve Growth Factors/chemistry
- Receptors, Nerve Growth Factor/chemistry
- Staining and Labeling