Site-specific recombinases: Molecular machines for the Genetic Revolution

Femi J. Olorunniji, Susan J. Rosser, W. Marshall Stark*

*Corresponding author for this work

Research output: Contribution to journalLiterature reviewpeer-review

Abstract / Description of output

The fields of molecular genetics, biotechnology and synthetic biology are demanding ever more sophisticated molecular tools for programmed precise modification of cell genomic DNA and other DNA sequences. This review presents the current state of knowledge and development of one important group of DNA-modifying enzymes, the site-specific recombinases (SSRs). SSRs are Nature's 'molecular machines' for cut-and-paste editing of DNA molecules by inserting, deleting or inverting precisely defined DNA segments. We survey the SSRs that have been put to use, and the types of applications for which they are suitable. We also discuss problems associated with uses of SSRs, how these problems can be minimized, and how recombinases are being re-engineered for improved performance and novel applications.

Original languageEnglish
Pages (from-to)673-684
Number of pages12
JournalBiochemical Journal
Issue number6
Publication statusPublished - 15 Mar 2016

Keywords / Materials (for Non-textual outputs)

  • genome engineering
  • recombinase
  • RMCE
  • site-specific recombination
  • synthetic biology


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