Sixteen new simple sequence repeat markers from Brassica juncea expressed sequences and their cross-species amplification

Clare J. Hopkins, Noel O.I. Cogan, Melanie Hand, Erica Jewell, Jatinder Kaur, Xi Li, Geraldine A.C. Lim, Alison E. Ling, Christopher Love, Hayley Mountford, Marija Todorovic, Megan Vardy, German C. Spangenberg, David Edwards, Jacqueline Batley*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The availability of expressed sequence data derived from gene discovery programs enables mining for simple sequence repeats (SSR), providing useful genetic markers for crop improvement. These markers are inexpensive, require minimal labour to produce and can frequently be associated with functionally annotated genes. This study presents the development and characterization of 16 expressed sequence tags (EST)-SSR markers from Brassica juncea and their cross-amplification across Brassica species. Sixteen primer pairs were assessed for polymorphism in all genomes of the diploid and amphidiploid Brassica species. The markers show reliable amplification, considerable polymorphism and high transferability across species, demonstrating the utility of EST-SSRs for genetic analysis of brassicas.
Original languageEnglish
Pages (from-to)697-700
Number of pages4
JournalMolecular Ecology Notes
Volume7
Issue number4
Early online date24 Jan 2007
DOIs
Publication statusPublished - Jul 2007

Keywords / Materials (for Non-textual outputs)

  • Brassica
  • cross-amplification
  • ESTs
  • genome specificity
  • Indian mustard
  • simple sequence repeats

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