Small molecule phosphorescent probes for O-2 imaging in 3D tissue models

Ruslan I. Dmitriev*, Alina V. Kondrashina, Klaus Koren, Ingo Klimant, Alexander V. Zhdanov, Janelle M. P. Pakan, Kieran W. McDermott, Dmitri B. Papkovsky

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Monitoring of oxygenation is important for physiological experiments investigating the growth, differentiation and function of individual cells in 3D tissue models. Phosphorescence based O-2 sensing and imaging potentially allow this task; however, current probes do not provide the desired bio-distribution and analytical performance. We present several new cell-penetrating phosphorescent conjugates of a Pt(II)-tetrakis(pentafluorophenyl) porphine (PtPFPP) dye produced by click-modification with thiols, and perform their evaluation as O-2 imaging probes for 3D tissue models. The hydrophilic glucose (Pt-Glc) and galactose (Pt-Gal) conjugates demonstrated minimal aggregation and self-quenching in aqueous media, and efficient in-depth staining of different cell types and multi-cellular aggregates at working concentrations

Original languageEnglish
Pages (from-to)853-866
Number of pages14
JournalBiomaterials science
Volume2
Issue number6
DOIs
Publication statusPublished - 2014

Keywords

  • ORGANOTYPIC SLICE CULTURES
  • STEM-CELL BIOLOGY
  • IN-VITRO
  • INTRACELLULAR OXYGEN
  • TUMOR HYPOXIA
  • CANCER-CELLS
  • BLOOD-FLOW
  • NANOPARTICLES
  • PORPHYRIN
  • PEPTIDES

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