Snail and Slug, key regulators of TGF-beta-induced EMT, are sufficient for the induction of single-cell invasion

Hildegonda P. H. Naber; Yvette Drabsch; B. Ewa Snaar-Jagalska; Peter ten Dijke; Theo van Laar

doi:10.1016/j.bbrc.2013.04.037

Snail and Slug, key regulators of TGF-beta-induced EMT, are sufficient for the induction of single-cell invasion

Hildegonda P. H. Naber, Yvette Drabsch, B. Ewa Snaar-Jagalska, Peter ten Dijke^*, Theo van Laar

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

TGF-beta plays a dual role in cancer; in early stages it inhibits tumor growth, whereas later it promotes invasion and metastasis. TGF-beta is thought to be pro-invasive by inducing epithelial-to-mesenchymal transition (EMT) via induction of transcriptional repressors, including Slug and Snail.

In this study, we investigated the role of Snail and Slug in TGF-beta-induced invasion in an in vitro invasion assay and in an embryonic zebrafish xenograft model. Ectopic expression of Slug or Snail promoted invasion of single, rounded amoeboid cells in vitro. In an embryonic zebrafish xenograft model, forced expression of Slug and Snail promoted single cell invasion and metastasis. Slug and Snail are sufficient for the induction of single-cell invasion in an in vitro invasion assay and in an embryonic zebrafish xenograft model. (C) 2013 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	58-63
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	435
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2013.04.037
Publication status	Published - 24 May 2013

Keywords / Materials (for Non-textual outputs)

TGF-beta
Single cell invasion
EMT
Slug
Snail
EPITHELIAL-MESENCHYMAL TRANSITION
BREAST-CANCER CELLS
GROWTH-FACTOR-BETA
ACTIN CYTOSKELETON
BONE METASTASIS
UP-REGULATION
TUMOR
EXPRESSION
ANGIOGENESIS
PROTEOLYSIS

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@article{c355920e70ac4d9db88ad6f69614cfbf,

title = "Snail and Slug, key regulators of TGF-beta-induced EMT, are sufficient for the induction of single-cell invasion",

abstract = "TGF-beta plays a dual role in cancer; in early stages it inhibits tumor growth, whereas later it promotes invasion and metastasis. TGF-beta is thought to be pro-invasive by inducing epithelial-to-mesenchymal transition (EMT) via induction of transcriptional repressors, including Slug and Snail.In this study, we investigated the role of Snail and Slug in TGF-beta-induced invasion in an in vitro invasion assay and in an embryonic zebrafish xenograft model. Ectopic expression of Slug or Snail promoted invasion of single, rounded amoeboid cells in vitro. In an embryonic zebrafish xenograft model, forced expression of Slug and Snail promoted single cell invasion and metastasis. Slug and Snail are sufficient for the induction of single-cell invasion in an in vitro invasion assay and in an embryonic zebrafish xenograft model. (C) 2013 Elsevier Inc. All rights reserved.",

keywords = "TGF-beta, Single cell invasion, EMT, Slug, Snail, EPITHELIAL-MESENCHYMAL TRANSITION, BREAST-CANCER CELLS, GROWTH-FACTOR-BETA, ACTIN CYTOSKELETON, BONE METASTASIS, UP-REGULATION, TUMOR, EXPRESSION, ANGIOGENESIS, PROTEOLYSIS",

author = "Naber, {Hildegonda P. H.} and Yvette Drabsch and Snaar-Jagalska, {B. Ewa} and {ten Dijke}, Peter and {van Laar}, Theo",

year = "2013",

month = may,

day = "24",

doi = "10.1016/j.bbrc.2013.04.037",

language = "English",

volume = "435",

pages = "58--63",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press",

number = "1",

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TY - JOUR

T1 - Snail and Slug, key regulators of TGF-beta-induced EMT, are sufficient for the induction of single-cell invasion

AU - Naber, Hildegonda P. H.

AU - Drabsch, Yvette

AU - Snaar-Jagalska, B. Ewa

AU - ten Dijke, Peter

AU - van Laar, Theo

PY - 2013/5/24

Y1 - 2013/5/24

N2 - TGF-beta plays a dual role in cancer; in early stages it inhibits tumor growth, whereas later it promotes invasion and metastasis. TGF-beta is thought to be pro-invasive by inducing epithelial-to-mesenchymal transition (EMT) via induction of transcriptional repressors, including Slug and Snail.In this study, we investigated the role of Snail and Slug in TGF-beta-induced invasion in an in vitro invasion assay and in an embryonic zebrafish xenograft model. Ectopic expression of Slug or Snail promoted invasion of single, rounded amoeboid cells in vitro. In an embryonic zebrafish xenograft model, forced expression of Slug and Snail promoted single cell invasion and metastasis. Slug and Snail are sufficient for the induction of single-cell invasion in an in vitro invasion assay and in an embryonic zebrafish xenograft model. (C) 2013 Elsevier Inc. All rights reserved.

AB - TGF-beta plays a dual role in cancer; in early stages it inhibits tumor growth, whereas later it promotes invasion and metastasis. TGF-beta is thought to be pro-invasive by inducing epithelial-to-mesenchymal transition (EMT) via induction of transcriptional repressors, including Slug and Snail.In this study, we investigated the role of Snail and Slug in TGF-beta-induced invasion in an in vitro invasion assay and in an embryonic zebrafish xenograft model. Ectopic expression of Slug or Snail promoted invasion of single, rounded amoeboid cells in vitro. In an embryonic zebrafish xenograft model, forced expression of Slug and Snail promoted single cell invasion and metastasis. Slug and Snail are sufficient for the induction of single-cell invasion in an in vitro invasion assay and in an embryonic zebrafish xenograft model. (C) 2013 Elsevier Inc. All rights reserved.

KW - TGF-beta

KW - Single cell invasion

KW - EMT

KW - Slug

KW - Snail

KW - EPITHELIAL-MESENCHYMAL TRANSITION

KW - BREAST-CANCER CELLS

KW - GROWTH-FACTOR-BETA

KW - ACTIN CYTOSKELETON

KW - BONE METASTASIS

KW - UP-REGULATION

KW - TUMOR

KW - EXPRESSION

KW - ANGIOGENESIS

KW - PROTEOLYSIS

U2 - 10.1016/j.bbrc.2013.04.037

DO - 10.1016/j.bbrc.2013.04.037

M3 - Article

SN - 0006-291X

VL - 435

SP - 58

EP - 63

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Snail and Slug, key regulators of TGF-beta-induced EMT, are sufficient for the induction of single-cell invasion

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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