SNP discovery in the region responsible For ETECF4ab/ac resistance in pigs

M.J. Jacobsen, A. Archibald, S. Cirera, I. Edfors, L. Andersson, M. Fredholm, C.B. Jorgensen

Research output: Contribution to conferenceAbstract

Abstract

Infection of the small intestine by enterotoxigenic Escherichia coli (ETEC) F4ab/ac is responsible for approximately 170,000 deaths in Danish pig production each year and thus constitutes an extreme welfare problem. Natural resistance towards this infection is inherited as a Mendelian recessive trait, and a SNP segregating with susceptibility/resistance identified by our group in MUC4 is presently used in a selection program by the Danish pig breeding industry. To elucidate the molecular mechanisms involved in ETECF4ab/ac susceptibility in pigs, we have established a detailed haplotype map of the porcine candidate region 13q41. This region covers approximately 3,5 Mb as determined by comparative mapping to human chromosome 3. The material used for the study is a three-generation family (total 236 pigs), where the founders are two Wild boars and eight Large White sows. All pigs have been phenotyped using an adhesion assay and their haplotypes are known from segregation analysis using flanking markers. By a targeted approach, the candidate region was subjected to SNP screening focusing on intronic sequences. A total of 24 genes were partially sequenced and SNPs were identified in GP5, CENTB2, APOD, PCYT1A, OSTα, ZDHHC19, TFRC, ACK1, MUC4, MUC20, KIAA0226, and LRCH3.Overall, 177 SNPs were discovered in the founder generation. Analysis shows a large conserved haplotype block around MUC4 on the F4ab/ac susceptible chromosomes. This detailed haplotype map serves as a valuable resource for further fine mapping of the locus involved in ETECF4ab/ac susceptibility, and ultimately for the identification of the causal mutation.
Original languageEnglish
Pagesabstract P530
Publication statusPublished - 2009
Event Plant & Animal Genomes XVII Conference - San Diego, United States
Duration: 10 Jan 200914 Jan 2009

Conference

Conference Plant & Animal Genomes XVII Conference
Country/TerritoryUnited States
CitySan Diego
Period10/01/0914/01/09

Keywords

  • disease resistance Escherichia coli Pigs SNP

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