Sodium and potassium ATPase of the teleost fish Catostomus commersoni. Sequence, protein structure and evolutionary conservation of the alpha-subunit

C Schönrock, S D Morley, Y Okawara, K Lederis, D Richter

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The alpha-subunit of a Na+/K+ ATPase has been cloned by analysing a lambda gt11 library constructed from polyA+ RNA from the hypothalamic region of the teleost fish Catostomus commersoni (white sucker). The cDNA clone consists of 3853 bp and predicts a protein of 1027 amino-acid residues. Alignment of the sucker sequence with protein sequences previously published for alpha-subunits from various species reveals a high degree of homology throughout the entire sequence containing five potential sites for N-glycosylation, a phosphorylation site and a site for binding fluorescein 5'-isothiocyanate (FITC). A hydropathy profile predicts a secondary structure of the Na+/K+ ATPase alpha-subunit with at least eight membrane-spanning domains. Northern and southern blot analyses suggest the existence of two distinct Na+/K+ ATPase alpha-subunit genes in the sucker genome.

Original languageEnglish
Pages (from-to)279-86
Number of pages8
JournalBiological chemistry Hoppe-Seyler
Volume372
Issue number4
Publication statusPublished - Apr 1991

Keywords / Materials (for Non-textual outputs)

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Biological Evolution
  • Cloning, Molecular
  • DNA
  • Fishes
  • Hypothalamus
  • Molecular Sequence Data
  • Protein Conformation
  • RNA
  • Sequence Alignment
  • Sodium-Potassium-Exchanging ATPase

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