Solution structure of CCP modules 10-12 illuminates functional architecture of the complement regulator, factor H

Elisavet Makou, Haydyn D T Mertens, Mateusz Maciejewski, Dinesh C Soares, Ilias Matis, Christoph Q Schmidt, Andrew P Herbert, Dmitri I Svergun, Paul N Barlow

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The 155-kDa plasma glycoprotein factor H (FH), which consists of 20 complement-control protein modules (CCPs), protects self-tissue but not foreign organisms from damage by the complement cascade. Protection is achieved by selective engagement of FH, via CCPs 1-4, CCPs 6-8 and CCPs 19-20, with polyanion-rich host surfaces that bear covalently attached, activation-specific, fragments of complement component C3. The role of intervening CCPs 9-18 in this process is obscured by lack of structural knowledge. We have concatenated new high-resolution solution structures of overlapping recombinant CCP pairs, 10-11 and 11-12, to form a three-dimensional structure of CCPs 10-12 and validated it by small-angle X-ray scattering of the recombinant triple module fragment. Superimposing CCP 12 of this 10-12 structure with CCP 12 from the previously solved CCPs 12-13 structure yielded an S-shaped structure for CCPs 10-13 in which modules are tilted by 80-110° with respect to immediate neighbours, but the bend between CCPs 10 and 11 is counter to the arc traced by CCPs 11-13. Including this four-CCP structure in interpretation of scattering data for the longer recombinant segments, CCPs 10-15 and 8-15, implied flexible attachment of CCPs 8-9 to CCP 10, but compact and intimate arrangements of CCP 14 with CCPs 12, 13 and 15. Taken together with difficulties in recombinant production of module pairs 13-14 and 14-15, the aberrant structure of CCP 13, and the variability of 13-14 linker sequences amongst orthologues, a structural dependency of CCP 14 on its neighbours is suggested; this has implications for the FH mechanism.
Original languageEnglish
Pages (from-to)295-312
JournalJournal of Molecular Biology
Volume 424
Issue number5
DOIs
Publication statusPublished - 2012

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