Spacer-free BODIPY fluorogens in antimicrobial peptides for direct imaging of fungal infection in human tissue

Lorena Mendive-Tapia, Can Zhao, Ahsan R. Akram, Sara Preciado, Fernando Albericio, Martin Lee, Alan Serrels, Nicola Kielland, Nick D Read, Rodolfo Lavilla, Marc Vendrell

Research output: Contribution to journalArticlepeer-review

Abstract

Fluorescent antimicrobial peptides are promising structures for in situ, real-time imaging of fungal infection. Here we report a fluorogenic probe to image Aspergillus fumigatus directly in human pulmonary tissue. We have developed a fluorogenic Trp-BODIPY amino acid with a spacer-free C-C linkage between Trp and a BODIPY fluorogen, which shows remarkable fluorescence enhancement in hydrophobic microenvironments. The incorporation of our fluorogenic amino acid in short antimicrobial peptides does not impair their selectivity for fungal cells, and enables rapid and direct fungal imaging without any washing steps. We have optimised the stability of our probes in human samples to perform multi-photon imaging of A. fumigatus in ex vivo human tissue. The incorporation of our unique BODIPY fluorogen in biologically-relevant peptides will accelerate the development of novel imaging probes with high sensitivity and specificity.
Original languageEnglish
Article number10940
Number of pages9
JournalNature Communications
Volume7
DOIs
Publication statusPublished - 9 Mar 2016

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