Spatial organization of the nucleus compartmentalizes and regulates the genome

Michael I. Robson, Andrea Rizzotto, Eric C. Schirmer*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract / Description of output

The nucleus must simultaneously orchestrate DNA replication, transcription, splicing, signalling, and directional transport of proteins into the nucleus and RNA out of the nucleus. Yet the nucleus has no internal membranes to compartmentalize these functions as the cytoplasm does. In fact, such compartmentalization would necessarily be detrimental because particular genes at different locations on the linear chromosomes need to be made at different times while others on the same chromosome need to be tightly shut off. Moreover, expressed genes need to be accessible to a feedback mechanism to determine when to modulate transcription. To accommodate these additional needs the nucleus appears to form microdomains by co-assembly of functional complexes. Thus, microdomains can either form around activated regions on a chromosome or regions on a linear chromosome could be fed into such microdomains for activation. Findings that genome encoded regulatory elements such as enhancers can be hundreds of kb and even Mb apart further highlights the need for such a system as these distal elements must come together in the 3D space of the genome for their efficient functioning. While this much is understood, there is much still to be learned about mechanisms that the nucleus uses to regulate the genome and much more to be learned about how these microdomains come into being. As there is no stable structure within the nucleus except for the nuclear envelope, much recent research has been focusing on potential roles of this subnuclear organelle in establishing 3D nuclear architecture and orchestrating the regulation of these various functions.

Original languageEnglish
Title of host publicationNuclear Pore Complexes in Genome Organization, Function and Maintenance
PublisherSpringer International Publishing Switzerland
Pages1-34
Number of pages34
ISBN (Electronic)9783319716145
ISBN (Print)9783319716121
DOIs
Publication statusPublished - 3 Feb 2018

Keywords / Materials (for Non-textual outputs)

  • gene position
  • genome organization
  • NET
  • nuclear envelope

Fingerprint

Dive into the research topics of 'Spatial organization of the nucleus compartmentalizes and regulates the genome'. Together they form a unique fingerprint.

Cite this