Spatial, temporal and molecular dynamics of swine influenza virus-specific CD8 tissue resident memory T cells

Veronica Martini; Matthew Edmans; Simon Gubbins; Siddharth Jayaraman; Basudev Paudyal; Sophie Morgan; Adam McNee; Théo Morin; Pramila Rijal; Wilhelm Gerner; Andrew K Sewell; Ryo Inoue; Mick Bailey; Timothy Connelley; Bryan Charleston; Alain Townsend; Peter Beverley; Elma Tchilian

doi:10.1038/s41385-021-00478-4

Spatial, temporal and molecular dynamics of swine influenza virus-specific CD8 tissue resident memory T cells

Veronica Martini, Matthew Edmans, Simon Gubbins, Siddharth Jayaraman, Basudev Paudyal, Sophie Morgan, Adam McNee, Théo Morin, Pramila Rijal, Wilhelm Gerner, Andrew K Sewell, Ryo Inoue, Mick Bailey, Timothy Connelley, Bryan Charleston, Alain Townsend, Peter Beverley, Elma Tchilian

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

For the first time we have defined naïve, central memory, effector memory and differentiated effector porcine CD8 T cells and analyzed their distribution in lymphoid and respiratory tissues after influenza infection or immunization, using peptide-MHC tetramers of three influenza nucleoprotein (NP) epitopes. The hierarchy of response to the three epitopes changes during the response in different tissues. Most NP-specific CD8 T cells in broncho-alveolar lavage (BAL) and lung are tissue resident memory cells (TRM) that express CD69 and downregulate CD45RA and CCR7. NP-specific cells isolated from BAL express genes characteristic of TRM, but gene expression differs at 7, 21 and 63 days post infection. In all tissues the frequency of NP-specific CD8 cells declines over 63 days almost to background levels but is best maintained in BAL. The kinetic of influenza specific memory CD8 T cell in this natural host species differs from that in small animal models.

Original language	English
Pages (from-to)	428-442
Journal	Mucosal Immunology
Volume	15
Issue number	3
Early online date	10 Feb 2022
DOIs	https://doi.org/10.1038/s41385-021-00478-4
Publication status	Published - Mar 2022

Keywords / Materials (for Non-textual outputs)

Animals
CD8-Positive T-Lymphocytes
Epitopes
Humans
Immunologic Memory
Influenza A virus
Influenza, Human
Memory T Cells
Molecular Dynamics Simulation
Orthomyxoviridae Infections
Swine

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10.1038/s41385-021-00478-4

s41385-021-00478-4Final published version, 3.85 MBLicence: Creative Commons: Attribution (CC-BY)

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Martini, V., Edmans, M., Gubbins, S., Jayaraman, S., Paudyal, B., Morgan, S., McNee, A., Morin, T., Rijal, P., Gerner, W., Sewell, A. K., Inoue, R., Bailey, M., Connelley, T., Charleston, B., Townsend, A., Beverley, P., & Tchilian, E. (2022). Spatial, temporal and molecular dynamics of swine influenza virus-specific CD8 tissue resident memory T cells. Mucosal Immunology, 15(3), 428-442. https://doi.org/10.1038/s41385-021-00478-4

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title = "Spatial, temporal and molecular dynamics of swine influenza virus-specific CD8 tissue resident memory T cells",

abstract = "For the first time we have defined na{\"i}ve, central memory, effector memory and differentiated effector porcine CD8 T cells and analyzed their distribution in lymphoid and respiratory tissues after influenza infection or immunization, using peptide-MHC tetramers of three influenza nucleoprotein (NP) epitopes. The hierarchy of response to the three epitopes changes during the response in different tissues. Most NP-specific CD8 T cells in broncho-alveolar lavage (BAL) and lung are tissue resident memory cells (TRM) that express CD69 and downregulate CD45RA and CCR7. NP-specific cells isolated from BAL express genes characteristic of TRM, but gene expression differs at 7, 21 and 63 days post infection. In all tissues the frequency of NP-specific CD8 cells declines over 63 days almost to background levels but is best maintained in BAL. The kinetic of influenza specific memory CD8 T cell in this natural host species differs from that in small animal models.",

keywords = "Animals, CD8-Positive T-Lymphocytes, Epitopes, Humans, Immunologic Memory, Influenza A virus, Influenza, Human, Memory T Cells, Molecular Dynamics Simulation, Orthomyxoviridae Infections, Swine",

author = "Veronica Martini and Matthew Edmans and Simon Gubbins and Siddharth Jayaraman and Basudev Paudyal and Sophie Morgan and Adam McNee and Th{\'e}o Morin and Pramila Rijal and Wilhelm Gerner and Sewell, {Andrew K} and Ryo Inoue and Mick Bailey and Timothy Connelley and Bryan Charleston and Alain Townsend and Peter Beverley and Elma Tchilian",

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doi = "10.1038/s41385-021-00478-4",

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Martini, V, Edmans, M, Gubbins, S, Jayaraman, S, Paudyal, B, Morgan, S, McNee, A, Morin, T, Rijal, P, Gerner, W, Sewell, AK, Inoue, R, Bailey, M, Connelley, T, Charleston, B, Townsend, A, Beverley, P & Tchilian, E 2022, 'Spatial, temporal and molecular dynamics of swine influenza virus-specific CD8 tissue resident memory T cells', Mucosal Immunology, vol. 15, no. 3, pp. 428-442. https://doi.org/10.1038/s41385-021-00478-4

TY - JOUR

T1 - Spatial, temporal and molecular dynamics of swine influenza virus-specific CD8 tissue resident memory T cells

AU - Martini, Veronica

AU - Edmans, Matthew

AU - Gubbins, Simon

AU - Jayaraman, Siddharth

AU - Paudyal, Basudev

AU - Morgan, Sophie

AU - McNee, Adam

AU - Morin, Théo

AU - Rijal, Pramila

AU - Gerner, Wilhelm

AU - Sewell, Andrew K

AU - Inoue, Ryo

AU - Bailey, Mick

AU - Connelley, Timothy

AU - Charleston, Bryan

AU - Townsend, Alain

AU - Beverley, Peter

AU - Tchilian, Elma

PY - 2022/3

Y1 - 2022/3

N2 - For the first time we have defined naïve, central memory, effector memory and differentiated effector porcine CD8 T cells and analyzed their distribution in lymphoid and respiratory tissues after influenza infection or immunization, using peptide-MHC tetramers of three influenza nucleoprotein (NP) epitopes. The hierarchy of response to the three epitopes changes during the response in different tissues. Most NP-specific CD8 T cells in broncho-alveolar lavage (BAL) and lung are tissue resident memory cells (TRM) that express CD69 and downregulate CD45RA and CCR7. NP-specific cells isolated from BAL express genes characteristic of TRM, but gene expression differs at 7, 21 and 63 days post infection. In all tissues the frequency of NP-specific CD8 cells declines over 63 days almost to background levels but is best maintained in BAL. The kinetic of influenza specific memory CD8 T cell in this natural host species differs from that in small animal models.

AB - For the first time we have defined naïve, central memory, effector memory and differentiated effector porcine CD8 T cells and analyzed their distribution in lymphoid and respiratory tissues after influenza infection or immunization, using peptide-MHC tetramers of three influenza nucleoprotein (NP) epitopes. The hierarchy of response to the three epitopes changes during the response in different tissues. Most NP-specific CD8 T cells in broncho-alveolar lavage (BAL) and lung are tissue resident memory cells (TRM) that express CD69 and downregulate CD45RA and CCR7. NP-specific cells isolated from BAL express genes characteristic of TRM, but gene expression differs at 7, 21 and 63 days post infection. In all tissues the frequency of NP-specific CD8 cells declines over 63 days almost to background levels but is best maintained in BAL. The kinetic of influenza specific memory CD8 T cell in this natural host species differs from that in small animal models.

KW - Animals

KW - CD8-Positive T-Lymphocytes

KW - Epitopes

KW - Humans

KW - Immunologic Memory

KW - Influenza A virus

KW - Influenza, Human

KW - Memory T Cells

KW - Molecular Dynamics Simulation

KW - Orthomyxoviridae Infections

KW - Swine

U2 - 10.1038/s41385-021-00478-4

DO - 10.1038/s41385-021-00478-4

M3 - Article

C2 - 35145208

SN - 1933-0219

VL - 15

SP - 428

EP - 442

JO - Mucosal Immunology

JF - Mucosal Immunology

IS - 3

ER -

Spatial, temporal and molecular dynamics of swine influenza virus-specific CD8 tissue resident memory T cells

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Keywords / Materials (for Non-textual outputs)

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