Species specific differences in use of ANP32 proteins by influenza A virus

Jason S. Long; Alewo Idoko-Akoh; Bhakti Mistry; Daniel H.  Goldhill; Ecco Staller; Jocelyn Schreyer; Craig Ross; Steve Goodbourn; Holly Shelton; Michael A Skinner; Helen Sang; Mike McGrew; Wendy S. Barclay

doi:10.7554/eLife.45066

Species specific differences in use of ANP32 proteins by influenza A virus

Jason S. Long, Alewo Idoko-Akoh, Bhakti Mistry, Daniel H. Goldhill, Ecco Staller , Jocelyn Schreyer, Craig Ross, Steve Goodbourn, Holly Shelton, Michael A Skinner, Helen Sang, Mike McGrew, Wendy S. Barclay

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

Influenza A viruses (IAV) are subject to species barriers that prevent frequent zoonotic transmission and pandemics. One of these barriers is the poor activity of avian IAV polymerases in human cells. Differences between avian and mammalian ANP32 proteins underlie this host range barrier. Human
ANP32A and ANP32B homologues both support function of human-adapted influenza polymerase but do not support efficient activity of avian IAV polymerase which requires avian ANP32A. We show here that the gene currently designated as avian ANP32B is evolutionarily distinct from mammalian ANP32B, and that chicken ANP32B does not support IAV polymerase activity even of human-adapted viruses. Consequently, IAV relies solely on chicken ANP32A to support its replication in chicken cells. Amino acids 129I and 130N, accounted for the inactivity of chicken ANP32B. Transfer of these residues to chicken ANP32A abolished support of IAV polymerase. Understanding ANP32 function will help develop antiviral strategies and aid the design of influenza virus resilient genome edited chickens.

Original language	English
Article number	2019;8:e45066
Number of pages	22
Journal	eLIFE
Volume	N/A
DOIs	https://doi.org/10.7554/eLife.45066
Publication status	Published - 4 Jun 2019

Keywords / Materials (for Non-textual outputs)

Influenza
polymerase
genome editing
disease
ANP32A
ANP32B
host pathogen interaction

Access to Document

10.7554/eLife.45066

Species specific differences in use of ...
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ISP 3 2017/22 Improving Animal Health and Welfare
Meddle, S.
BBSRC
1/04/17 → 31/03/22
Project: Research
ISP 1 2017/22 Blueprints for Healthy Animals
Watson, M.
BBSRC
1/04/17 → 31/03/22
Project: Research
Master Agreement between Cobb-Vantress inc and University of Edinburgh
Whitelaw, B.
Non-EU other
13/12/13 → 12/12/22
Project: Research

Mike McGrew
- mike.mcgrewroslin.ed.acuk
- Royal (Dick) School of Veterinary Studies - Personal Chair of Avian Reproductive Technologies
Person: Academic: Research Active

Cite this

@article{d15d090c42ea4e5791718881f889b36f,

title = "Species specific differences in use of ANP32 proteins by influenza A virus",

abstract = "Influenza A viruses (IAV) are subject to species barriers that prevent frequent zoonotic transmission and pandemics. One of these barriers is the poor activity of avian IAV polymerases in human cells. Differences between avian and mammalian ANP32 proteins underlie this host range barrier. HumanANP32A and ANP32B homologues both support function of human-adapted influenza polymerase but do not support efficient activity of avian IAV polymerase which requires avian ANP32A. We show here that the gene currently designated as avian ANP32B is evolutionarily distinct from mammalian ANP32B, and that chicken ANP32B does not support IAV polymerase activity even of human-adapted viruses. Consequently, IAV relies solely on chicken ANP32A to support its replication in chicken cells. Amino acids 129I and 130N, accounted for the inactivity of chicken ANP32B. Transfer of these residues to chicken ANP32A abolished support of IAV polymerase. Understanding ANP32 function will help develop antiviral strategies and aid the design of influenza virus resilient genome edited chickens.",

keywords = "Influenza, polymerase, genome editing, disease, ANP32A, ANP32B, host pathogen interaction",

author = "Long, {Jason S.} and Alewo Idoko-Akoh and Bhakti Mistry and Goldhill, {Daniel H.} and Ecco Staller and Jocelyn Schreyer and Craig Ross and Steve Goodbourn and Holly Shelton and Skinner, {Michael A} and Helen Sang and Mike McGrew and Barclay, {Wendy S.}",

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doi = "10.7554/eLife.45066",

language = "English",

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TY - JOUR

T1 - Species specific differences in use of ANP32 proteins by influenza A virus

AU - Long, Jason S.

AU - Idoko-Akoh, Alewo

AU - Mistry, Bhakti

AU - Goldhill, Daniel H.

AU - Staller , Ecco

AU - Schreyer, Jocelyn

AU - Ross, Craig

AU - Goodbourn, Steve

AU - Shelton, Holly

AU - Skinner, Michael A

AU - Sang, Helen

AU - McGrew, Mike

AU - Barclay, Wendy S.

PY - 2019/6/4

Y1 - 2019/6/4

N2 - Influenza A viruses (IAV) are subject to species barriers that prevent frequent zoonotic transmission and pandemics. One of these barriers is the poor activity of avian IAV polymerases in human cells. Differences between avian and mammalian ANP32 proteins underlie this host range barrier. HumanANP32A and ANP32B homologues both support function of human-adapted influenza polymerase but do not support efficient activity of avian IAV polymerase which requires avian ANP32A. We show here that the gene currently designated as avian ANP32B is evolutionarily distinct from mammalian ANP32B, and that chicken ANP32B does not support IAV polymerase activity even of human-adapted viruses. Consequently, IAV relies solely on chicken ANP32A to support its replication in chicken cells. Amino acids 129I and 130N, accounted for the inactivity of chicken ANP32B. Transfer of these residues to chicken ANP32A abolished support of IAV polymerase. Understanding ANP32 function will help develop antiviral strategies and aid the design of influenza virus resilient genome edited chickens.

AB - Influenza A viruses (IAV) are subject to species barriers that prevent frequent zoonotic transmission and pandemics. One of these barriers is the poor activity of avian IAV polymerases in human cells. Differences between avian and mammalian ANP32 proteins underlie this host range barrier. HumanANP32A and ANP32B homologues both support function of human-adapted influenza polymerase but do not support efficient activity of avian IAV polymerase which requires avian ANP32A. We show here that the gene currently designated as avian ANP32B is evolutionarily distinct from mammalian ANP32B, and that chicken ANP32B does not support IAV polymerase activity even of human-adapted viruses. Consequently, IAV relies solely on chicken ANP32A to support its replication in chicken cells. Amino acids 129I and 130N, accounted for the inactivity of chicken ANP32B. Transfer of these residues to chicken ANP32A abolished support of IAV polymerase. Understanding ANP32 function will help develop antiviral strategies and aid the design of influenza virus resilient genome edited chickens.

KW - Influenza

KW - polymerase

KW - genome editing

KW - disease

KW - ANP32A

KW - ANP32B

KW - host pathogen interaction

U2 - 10.7554/eLife.45066

DO - 10.7554/eLife.45066

M3 - Article

SN - 2050-084X

VL - N/A

JO - eLIFE

JF - eLIFE

M1 - 2019;8:e45066

ER -

Species specific differences in use of ANP32 proteins by influenza A virus

Abstract

Keywords / Materials (for Non-textual outputs)

Access to Document

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Projects

ISP 3 2017/22 Improving Animal Health and Welfare

ISP 1 2017/22 Blueprints for Healthy Animals

Master Agreement between Cobb-Vantress inc and University of Edinburgh

Profiles

Mike McGrew

Cite this