Specific interactions between transcription factors and the promoter-regulatory region of the human cytomegalovirus major immediate-early gene

P Ghazal, H Lubon, L Hennighausen

Research output: Contribution to journalArticlepeer-review

Abstract

Repeat sequence motifs as well as unique sequences between nucleotides -150 and -22 of the human cytomegalovirus immediate-early 1 gene interact in vitro with nuclear proteins. We show that a transcriptional element between nucleotides -91 and -65 stimulated promoter activity in vivo and in vitro by binding specific cellular transcription factors. Finally, a common sequence motif, (T)TGG/AC, present in 15 of the determined binding sites suggests a particular class of nuclear factors associated with the immediate-early 1 gene.
Original languageEnglish
Pages (from-to)1076-9
Number of pages4
JournalJournal of Virology
Volume62
Issue number3
Publication statusPublished - 1988

Keywords

  • Base Sequence
  • Binding Sites
  • Cytomegalovirus
  • DNA, Viral
  • Enhancer Elements, Genetic
  • Gene Products, tat
  • Genes, Regulator
  • Genes, Viral
  • HeLa Cells
  • Humans
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • Repetitive Sequences, Nucleic Acid
  • Transcription Factors

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