Specificity of lymphoreticular accumulation of prion protein for variant Creutzfeldt-Jakob disease

D A Hilton; J Sutak; M E F Smith; M Penney; L Conyers; P Edwards; L McCardle; D Ritchie; M W Head; C A Wiley; J W Ironside

doi:10.1136/jcp.2003.012278

Specificity of lymphoreticular accumulation of prion protein for variant Creutzfeldt-Jakob disease

D A Hilton, J Sutak, M E F Smith, M Penney, L Conyers, P Edwards, L McCardle, D Ritchie, M W Head, C A Wiley, J W Ironside

Research output: Contribution to journal › Article › peer-review

Abstract

BACKGROUND: Immunocytochemical accumulation of prion protein (PrP) in lymphoid tissues is a feature of variant Creutzfeldt-Jakob disease (vCJD) that has been used both to aid in the diagnosis of patients and as a basis of large scale screening studies to assess the prevalence of preclinical disease in the UK. However, the specificity of this approach is unknown.

AIM: To assess the specificity of lymphoreticular accumulation of PrP for vCJD by examining a range of human diseases.

METHODS: Paraffin wax embedded lymphoreticular tissues from patients with several reactive conditions (58 cases), tumours (27 cases), vCJD (54 cases), and other human prion diseases (56 cases) were assessed. PrP accumulation was assessed by immunocytochemistry using two different monoclonal anti-PrP antibodies and a sensitive detection system.

RESULTS: All cases of vCJD showed widespread lymphoreticular accumulation of PrP; however, this was not seen in the other conditions examined.

CONCLUSION: Lymphoreticular accumulation of PrP, as assessed by immunocytochemistry, appears to be a highly specific feature of vCJD.

Original language	English
Pages (from-to)	300-302
Number of pages	3
Journal	Journal of Clinical Pathology
Volume	57
Issue number	3
DOIs	https://doi.org/10.1136/jcp.2003.012278
Publication status	Published - Mar 2004

Keywords / Materials (for Non-textual outputs)

Blotting, Western
Creutzfeldt-Jakob Syndrome
Humans
Immunohistochemistry
Inflammation
Lymphoid Tissue
Mononuclear Phagocyte System
Neoplasms
PrPSc Proteins
Prion Diseases
Prions
Sensitivity and Specificity

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http://jcp.bmj.com/content/57/3/300.full.html

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title = "Specificity of lymphoreticular accumulation of prion protein for variant Creutzfeldt-Jakob disease",

abstract = "BACKGROUND: Immunocytochemical accumulation of prion protein (PrP) in lymphoid tissues is a feature of variant Creutzfeldt-Jakob disease (vCJD) that has been used both to aid in the diagnosis of patients and as a basis of large scale screening studies to assess the prevalence of preclinical disease in the UK. However, the specificity of this approach is unknown.AIM: To assess the specificity of lymphoreticular accumulation of PrP for vCJD by examining a range of human diseases.METHODS: Paraffin wax embedded lymphoreticular tissues from patients with several reactive conditions (58 cases), tumours (27 cases), vCJD (54 cases), and other human prion diseases (56 cases) were assessed. PrP accumulation was assessed by immunocytochemistry using two different monoclonal anti-PrP antibodies and a sensitive detection system.RESULTS: All cases of vCJD showed widespread lymphoreticular accumulation of PrP; however, this was not seen in the other conditions examined.CONCLUSION: Lymphoreticular accumulation of PrP, as assessed by immunocytochemistry, appears to be a highly specific feature of vCJD.",

keywords = "Blotting, Western, Creutzfeldt-Jakob Syndrome, Humans, Immunohistochemistry, Inflammation, Lymphoid Tissue, Mononuclear Phagocyte System, Neoplasms, PrPSc Proteins, Prion Diseases, Prions, Sensitivity and Specificity",

author = "Hilton, {D A} and J Sutak and Smith, {M E F} and M Penney and L Conyers and P Edwards and L McCardle and D Ritchie and Head, {M W} and Wiley, {C A} and Ironside, {J W}",

year = "2004",

month = mar,

doi = "10.1136/jcp.2003.012278",

language = "English",

volume = "57",

pages = "300--302",

journal = "Journal of Clinical Pathology",

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TY - JOUR

T1 - Specificity of lymphoreticular accumulation of prion protein for variant Creutzfeldt-Jakob disease

AU - Hilton, D A

AU - Sutak, J

AU - Smith, M E F

AU - Penney, M

AU - Conyers, L

AU - Edwards, P

AU - McCardle, L

AU - Ritchie, D

AU - Head, M W

AU - Wiley, C A

AU - Ironside, J W

PY - 2004/3

Y1 - 2004/3

N2 - BACKGROUND: Immunocytochemical accumulation of prion protein (PrP) in lymphoid tissues is a feature of variant Creutzfeldt-Jakob disease (vCJD) that has been used both to aid in the diagnosis of patients and as a basis of large scale screening studies to assess the prevalence of preclinical disease in the UK. However, the specificity of this approach is unknown.AIM: To assess the specificity of lymphoreticular accumulation of PrP for vCJD by examining a range of human diseases.METHODS: Paraffin wax embedded lymphoreticular tissues from patients with several reactive conditions (58 cases), tumours (27 cases), vCJD (54 cases), and other human prion diseases (56 cases) were assessed. PrP accumulation was assessed by immunocytochemistry using two different monoclonal anti-PrP antibodies and a sensitive detection system.RESULTS: All cases of vCJD showed widespread lymphoreticular accumulation of PrP; however, this was not seen in the other conditions examined.CONCLUSION: Lymphoreticular accumulation of PrP, as assessed by immunocytochemistry, appears to be a highly specific feature of vCJD.

AB - BACKGROUND: Immunocytochemical accumulation of prion protein (PrP) in lymphoid tissues is a feature of variant Creutzfeldt-Jakob disease (vCJD) that has been used both to aid in the diagnosis of patients and as a basis of large scale screening studies to assess the prevalence of preclinical disease in the UK. However, the specificity of this approach is unknown.AIM: To assess the specificity of lymphoreticular accumulation of PrP for vCJD by examining a range of human diseases.METHODS: Paraffin wax embedded lymphoreticular tissues from patients with several reactive conditions (58 cases), tumours (27 cases), vCJD (54 cases), and other human prion diseases (56 cases) were assessed. PrP accumulation was assessed by immunocytochemistry using two different monoclonal anti-PrP antibodies and a sensitive detection system.RESULTS: All cases of vCJD showed widespread lymphoreticular accumulation of PrP; however, this was not seen in the other conditions examined.CONCLUSION: Lymphoreticular accumulation of PrP, as assessed by immunocytochemistry, appears to be a highly specific feature of vCJD.

KW - Blotting, Western

KW - Creutzfeldt-Jakob Syndrome

KW - Humans

KW - Immunohistochemistry

KW - Inflammation

KW - Lymphoid Tissue

KW - Mononuclear Phagocyte System

KW - Neoplasms

KW - PrPSc Proteins

KW - Prion Diseases

KW - Prions

KW - Sensitivity and Specificity

U2 - 10.1136/jcp.2003.012278

DO - 10.1136/jcp.2003.012278

M3 - Article

C2 - 14990604

SN - 0021-9746

VL - 57

SP - 300

EP - 302

JO - Journal of Clinical Pathology

JF - Journal of Clinical Pathology

IS - 3

ER -

Specificity of lymphoreticular accumulation of prion protein for variant Creutzfeldt-Jakob disease

Abstract

Keywords / Materials (for Non-textual outputs)

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