SpeedyGenesXL: An automated, high-throughput platform for the preparation of bespoke ultralarge variant libraries for directed evolution

Joanna C. Sadler, Neil Swainston, Mark S. Dunstan, Andrew Currin, Douglas B. Kell*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract / Description of output

Directed evolution of proteins is a highly effective strategy for tailoring biocatalysts to a particular application, and is capable of engineering improvements such as kcat, thermostability and organic solvent tolerance. It is recognized that large and systematic libraries are required to navigate a protein’s vast and rugged sequence landscape effectively, yet their preparation is nontrivial and commercial libraries are extremely costly. To address this, we have developed SpeedyGenesXL, an automated, high-throughput platform for the production of wild-type genes, Boolean OR, combinatorial, or combinatorial-OR-type libraries based on the SpeedyGenes methodology. Together this offers a flexible platform for library synthesis, capable of generating many different bespoke, diverse libraries simultaneously.

Original languageEnglish
Title of host publicationDirected Evolution
Subtitle of host publicationMethods and Protocols
PublisherHUMANA PRESS INC
Chapter5
Pages67-83
Number of pages17
Volume2461
ISBN (Electronic)978-1-0716-2152-3
ISBN (Print)978-1-0716-2151-6
DOIs
Publication statusE-pub ahead of print - 22 Jun 2022

Publication series

NameMethods in Molecular Biology
Volume2461
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords / Materials (for Non-textual outputs)

  • automation
  • bespoke gene variant library synthesis
  • directed evolution
  • synthetic biology

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