Splicing Factors Facilitate RNAi-Directed Silencing in Fission Yeast

Elizabeth Bayne, Manuela Portoso, Alexander Kagansky, Isabelle C. Kos-Braun, Takeshi Urano, Karl Ekwall, Flavia Alves, Juri Rappsilber, Robin C. Allshire

Research output: Contribution to journalArticlepeer-review

Abstract

Heterochromatin formation at fission yeast centromeres is directed by RNA interference (RNAi). Noncoding transcripts derived from centromeric repeats are processed into small interfering RNAs (siRNAs) that direct the RNA-induced transcriptional silencing (RITS) effector complex to engage centromer transcripts, resulting in recruitment of the histone H3 lysine 9 methyltransferase Clr4, and hence silencing. We have found that defects in specific splicing factors, but not splicing itself, affect the generation of centromeric siRNAs and consequently centromeric heterochromatin integrity. Moreover, splicing factors physically associate with Cid12, a component of the RNAi machinery, and with centromeric chromatin, consistent with a direct role in RNAi. We propose that spliceosomal complexes provide a platform for siRNA generation and hence facilitate effective centromere repeat silencing.

Original languageEnglish
Pages (from-to)602-606
Number of pages5
JournalScience
Volume322
Issue number5901
DOIs
Publication statusPublished - 24 Oct 2008

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