Stabilizing Hepatocellular Phenotype Using Optimized Synthetic Surfaces

Baltasar Lucendo-Villarin, Katherine Cameron, Dagmara Szkolnicka, Paul Travers, Ferdous Khan, Jeffrey G Walton, John Iredale, Mark Bradley, David C Hay

Research output: Contribution to journalArticlepeer-review

Abstract

Currently, one of the major limitations in cell biology is maintaining differentiated cell phenotype. Biological matrices are commonly used for culturing and maintaining primary and pluripotent stem cell derived hepatocytes. While biological matrices are useful, they permit short term culture of hepatocytes, limiting their widespread application. We have attempted to overcome the limitations using a synthetic polymer coating. Polymers represent one of the broadest classes of biomaterials and possess a wide range of mechanical, physical and chemical properties, which can be fine-tuned for purpose. Importantly, such materials can be scaled to quality assured standards and display batch-to-batch consistency. This is essential if cells are to be expanded for high through-put screening in the pharmaceutical testing industry or for cellular based therapy. Polyurethanes (PUs) are one group of materials that have shown promise in cell culture. Our recent progress in optimizing a polyurethane coated surface, for long-term culture of human hepatocytes displaying stable phenotype, is presented and discussed.

Original languageEnglish
Article numbere51723
JournalJournal of Visualized Experiments (JoVE)
Issue number91
DOIs
Publication statusPublished - 26 Sep 2014

Keywords

  • Pluripotent stem cell
  • polyurethane
  • polymer coating
  • p450 metabolism
  • stable phenotype
  • gamma irradiation
  • ultraviolet irradiation

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