Projects per year
Abstract
As redesigning organisms using engineering principles is one of the purposes of synthetic biology (SynBio), the standardization of experimental methods and DNA parts is becoming increasingly a necessity. The synthetic biology community focusing on the engineering of Saccharomyces cerevisiae has been in the foreground in this area, conceiving several well-characterized SynBio toolkits widely adopted by the community. In this review, the molecular methods and toolkits developed for S. cerevisiae are discussed in terms of their contributions to the required standardization efforts. In addition, the toolkits designed for emerging nonconventional yeast species including Yarrowia lipolytica, Komagataella phaffii, and Kluyveromyces marxianus are also reviewed. Without a doubt, the characterized DNA parts combined with the standardized assembly strategies highlighted in these toolkits have greatly contributed to the rapid development of many metabolic engineering and diagnostics applications among others. Despite the growing capacity in deploying synthetic biology for common yeast genome engineering works, the yeast community has a long journey to go to exploit it in more sophisticated and delicate applications like bioautomation.
Original language | English |
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Pages (from-to) | 2527-2547 |
Number of pages | 21 |
Journal | ACS Synthetic Biology |
Volume | 11 |
Early online date | 8 Aug 2022 |
DOIs | |
Publication status | Published - 19 Aug 2022 |
Keywords / Materials (for Non-textual outputs)
- automation
- biological parts
- characterization
- standardization
- synthetic biology
- yeast toolkits
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Dive into the research topics of 'Standardization of synthetic biology tools and assembly methods for Saccharomyces cerevisiae and emerging yeast species'. Together they form a unique fingerprint.Projects
- 2 Finished
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Metabolic Engineering of Yarrowia lipolytica to produce an advanced biofuel from cellulose and plant wastes
Rios Solis, L. (Principal Investigator)
UK central government bodies/local authorities, health and hospital authorities
1/08/20 → 30/03/23
Project: Research
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Dynamic regulation of mRNA processing in adapting fungi
Wallace, E. (Principal Investigator)
19/01/18 → 18/01/24
Project: Research