Steady-state cell-free gene expression with microfluidic chemostats

Nadanai Laohakunakorn, Barbora Lavickova, Zoe Swank, Julie Laurent, Sebastian J. Maerkl

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Cell-free synthetic biology offers an approach to building and testing gene circuits in a simplified environment free from the complexity of a living cell. Recent advances in microfluidic devices allowed cell-free reactions to run under nonequilibrium, steady-state conditions enabling the implementation of dynamic gene regulatory circuits in vitro. In this chapter, we present a detailed protocol to fabricate a microfluidic chemostat device which enables such an operation, detailing essential steps in photolithography, soft lithography, and hardware setup.
Original languageEnglish
Title of host publicationSynthetic Gene Circuits
Subtitle of host publicationMethods and Protocols
PublisherSpringer
Chapter9
Pages189-203
Number of pages14
Volume2229
ISBN (Electronic)978-1-0716-1032-9
ISBN (Print)978-1-0716-1031-2
DOIs
Publication statusPublished - 7 Jan 2021

Publication series

NameSynthetic Gene Circuits
Volume2229
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • microfluidics
  • cell-free
  • synthetic biology
  • steady state gene expression

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