Sterol content analysis suggests altered eburicol 14α-demethylase (CYP51) activity in isolates of Mycosphaerella graminicola adapted to azole fungicides

Tim P. Bean, Hans J. Cools, John A. Lucas, Nathaniel D. Hawkins, Jane L. Ward, Michael W. Shaw, Bart A. Fraaije

Research output: Contribution to journalArticlepeer-review

Abstract

The recent decline in the effectiveness of some azole fungicides in controlling the wheat pathogen Mycosphaerella graminicola has been associated with mutations in the CYP51 gene encoding the azole target, the eburicol 14α-demethylase (CYP51), an essential enzyme of the ergosterol biosynthesis pathway. In this study, analysis of the sterol content of M. graminicola isolates carrying different variants of the CYP51 gene has revealed quantitative differences in sterol intermediates, particularly the CYP51 substrate eburicol. Together with CYP51 gene expression studies, these data suggest that mutations in the CYP51 gene impact on the activity of the CYP51 protein.

Original languageEnglish
Pages (from-to)266-273
Number of pages8
JournalFEMS Microbiology Letters
Volume296
Issue number2
DOIs
Publication statusPublished - 1 Jul 2009

Keywords

  • Azole fungicide
  • CYP51
  • Eburicol 14α-demethylase
  • Septoria tritici
  • Sterol content

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