Projects per year
Dendritic spines are basic units of neuronal information processing and their structure is closely reflected in their function. Defects in synaptic development are common in neurodevelopmental disorders, making detailed knowledge of age-dependent changes in spine morphology essential for understanding disease mechanisms. However, little is known about the functionally important fine-morphological structures, such as spine necks, due to the limited spatial resolution of conventional light microscopy. Using stimulated emission depletion microscopy (STED), we examined spine morphology at the nanoscale during normal development in mice, and tested the hypothesis that it is impaired in a mouse model of fragile X syndrome (FXS). In contrast to common belief, we find that, in normal development, spine heads become smaller, while their necks become wider and shorter, indicating that synapse compartmentalization decreases substantially with age. In the mouse model of FXS, this developmental trajectory is largely intact, with only subtle differences that are dependent on age and brain region. Together, our findings challenge current dogmas of both normal spine development as well as spine dysgenesis in FXS, highlighting the importance of super-resolution imaging approaches for elucidating structure-function relationships of dendritic spines.
|Number of pages||8|
|Journal||The Journal of Neuroscience|
|Early online date||30 Apr 2014|
|Publication status||Published - 2014|
- DENDRITIC SPINES
- Fragile X Syndrome
- Spine dysgenesis
- super-resolution microscopy
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- 1 Finished
The neuropathophysiology associated with Syngap mutations: further evidence for an mGluR5 signaling axis in ID/ASD
Kind, P., Daw, M. & Wyllie, D.
1/03/13 → 31/08/16
- Deanery of Biomedical Sciences - Personal Chair of Developmental Neuroscience
- Centre for Discovery Brain Sciences
- Edinburgh Neuroscience
Person: Academic: Research Active