Stobadine-induced hastening of sensorimotor recovery after focal ischemia/reperfusion is associated with cerebrovascular protection

F. Plaisier, M. Bastide*, T. Ouk, O. Petrault, M. Laprais, S. Stolc, R. Bordet

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

In a model of 1 hour-intraluminal occlusion of rat middle cerebral artery (MCA), we investigated the spontaneous recovery of vascular functions and functional deficit together with ischemia volume evolution at 24 h, 3 days and 7 days of reperfusion. Infarct cerebral volumes and edema were quantified with histological methods. Endothelium-dependent and smooth muscle potassium inward rectifier current (Kir2.x)-dependent relaxing responses of MCA were tested using Halpern arteriograph and Kir2.x current density evaluated on MCA myocytes with whole-cell patch-clamp technique. Sensorimotor recovery was estimated according to performances obtained with adhesive removal test and prehensile traction test. A time-dependent improvement of smooth muscle K-dependent vasorelaxation and Kir2.x current density is observed at 7 days of reperfusion while endothelium-dependent relaxation is still impaired. In parallel a significant reduction of functional deficit is observed at 7 days of reperfusion together with a time-matched reduction of striatal infarct and edema volumes. Administration of an antioxidant agent, stobadine, at time of reperfusion and 5 h later allowed: (i) a neuroprotective effect with a significant reduction of infarct size compared to vehicle-treated rats; (ii) a prevention of endothelial-dependent relaxation and Kir2.x current density reductions of MCA ipsilateral to occlusion; (iii) a hastening of the functional recovery. The beneficial effect of stobadine underlines a link between vascular protection, neuronal protection and sensorimotor recovery that could become a promising pharmacological target in the treatment of cerebral ischemia. (C) 2008 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)240-249
Number of pages10
JournalBrain Research
Volume1208
DOIs
Publication statusPublished - 7 May 2008

Keywords

  • cerebral ischemia
  • neurological deficit
  • cerebral vessel
  • potassium channel
  • antioxidant agent
  • CEREBRAL-ARTERY OCCLUSION
  • BRAIN ISCHEMIC TOLERANCE
  • ENDOTHELIAL DYSFUNCTION
  • SMOOTH-MUSCLE
  • NITRIC-OXIDE
  • PYRIDOINDOLE STOBADINE
  • RAT-BRAIN
  • REPERFUSION
  • IMPAIRMENT
  • STROKE

Fingerprint

Dive into the research topics of 'Stobadine-induced hastening of sensorimotor recovery after focal ischemia/reperfusion is associated with cerebrovascular protection'. Together they form a unique fingerprint.

Cite this