TY - JOUR
T1 - Stomatal regulators are co-opted for seta development in the astomatous liverwort Marchantia polymorpha
AU - Moriya, Kenta C.
AU - Shirakawa, Makoto
AU - Loue-Manifel, Jeanne
AU - Matsuda, Yoriko
AU - Lu, Yen Ting
AU - Tamura, Kentaro
AU - Oka, Yoshito
AU - Matsushita, Tomonao
AU - Hara-Nishimura, Ikuko
AU - Ingram, Gwyneth
AU - Nishihama, Ryuichi
AU - Goodrich, Justin
AU - Kohchi, Takayuki
AU - Shimada, Tomoo
N1 - Funding Information:
We thank T. Nakagawa (Shimane University, Japan), S. Mano (National Institute for Basic Biology, Japan), S. S. Sugano (National Institute of Advanced Industrial Science and Technology, Japan) and K. U. Torii (The University of Texas at Austin, USA) for sharing the materials. We also thank K. Nakajima (Nara Institute of Science and Technology, Japan) for sharing the figures of plants in Fig. 5b. We are grateful to J. Raymond for his critical readings of this manuscript. This work was supported by Ministry of Education, Culture, Sports, Science and Technology/Japan Society for the Promotion of Science (MEXT/JSPS) KAKENHI grants to M.S. (JP19K06722 and JP20H05416), K.T. (JP26711017 and JP18K06283), Y.O. (JP18K19964), T.M. (JP20H05905 and JP20H05906), I.H.-N. (JP15H05776), R.N. (JP20H04884) and T.S. (JP18K06284); Grants-in-Aid JSPS Fellows to K.C.M. (JP21J14990) and M.S. (JP12J05453); and the Takeda Science Foundation, the Kato Memorial Bioscience Foundation and the Ohsumi Frontier Science Foundation to M.S. J.L.-M. and Y.-T.L. were supported by PhD studentships from the Darwin Trust of Edinburgh.
Funding Information:
We thank T. Nakagawa (Shimane University, Japan), S. Mano (National Institute for Basic Biology, Japan), S. S. Sugano (National Institute of Advanced Industrial Science and Technology, Japan) and K. U. Torii (The University of Texas at Austin, USA) for sharing the materials. We also thank K. Nakajima (Nara Institute of Science and Technology, Japan) for sharing the figures of plants in Fig. . We are grateful to J. Raymond for his critical readings of this manuscript. This work was supported by Ministry of Education, Culture, Sports, Science and Technology/Japan Society for the Promotion of Science (MEXT/JSPS) KAKENHI grants to M.S. (JP19K06722 and JP20H05416), K.T. (JP26711017 and JP18K06283), Y.O. (JP18K19964), T.M. (JP20H05905 and JP20H05906), I.H.-N. (JP15H05776), R.N. (JP20H04884) and T.S. (JP18K06284); Grants-in-Aid JSPS Fellows to K.C.M. (JP21J14990) and M.S. (JP12J05453); and the Takeda Science Foundation, the Kato Memorial Bioscience Foundation and the Ohsumi Frontier Science Foundation to M.S. J.L.-M. and Y.-T.L. were supported by PhD studentships from the Darwin Trust of Edinburgh.
Publisher Copyright:
© 2023, The Author(s), under exclusive licence to Springer Nature Limited.
PY - 2023/2/1
Y1 - 2023/2/1
N2 - The evolution of special types of cells requires the acquisition of new gene regulatory networks controlled by transcription factors (TFs). In stomatous plants, a TF module formed by subfamilies Ia and IIIb basic helix–loop–helix TFs (Ia-IIIb bHLH) regulates stomatal formation; however, how this module evolved during land plant diversification remains unclear. Here we show that, in the astomatous liverwort Marchantia polymorpha, a Ia-IIIb bHLH module regulates the development of a unique sporophyte tissue, the seta, which is found in mosses and liverworts. The sole Ia bHLH gene, MpSETA, and a IIIb bHLH gene, MpICE2, regulate the cell division and/or differentiation of seta lineage cells. MpSETA can partially replace the stomatal function of Ia bHLH TFs in Arabidopsis thaliana, suggesting that a common regulatory mechanism underlies setal and stomatal formation. Our findings reveal the co-option of a Ia-IIIb bHLH TF module for regulating cell fate determination and/or cell division of distinct types of cells during land plant evolution.
AB - The evolution of special types of cells requires the acquisition of new gene regulatory networks controlled by transcription factors (TFs). In stomatous plants, a TF module formed by subfamilies Ia and IIIb basic helix–loop–helix TFs (Ia-IIIb bHLH) regulates stomatal formation; however, how this module evolved during land plant diversification remains unclear. Here we show that, in the astomatous liverwort Marchantia polymorpha, a Ia-IIIb bHLH module regulates the development of a unique sporophyte tissue, the seta, which is found in mosses and liverworts. The sole Ia bHLH gene, MpSETA, and a IIIb bHLH gene, MpICE2, regulate the cell division and/or differentiation of seta lineage cells. MpSETA can partially replace the stomatal function of Ia bHLH TFs in Arabidopsis thaliana, suggesting that a common regulatory mechanism underlies setal and stomatal formation. Our findings reveal the co-option of a Ia-IIIb bHLH TF module for regulating cell fate determination and/or cell division of distinct types of cells during land plant evolution.
U2 - 10.1038/s41477-022-01325-5
DO - 10.1038/s41477-022-01325-5
M3 - Article
AN - SCOPUS:85146580748
SN - 2055-026X
VL - 9
SP - 302
EP - 314
JO - Nature Plants
JF - Nature Plants
ER -